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首页> 外文会议>Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II; 20040127-20040128; San Jose,CA; US >Lanthanide Enhanced Luminescence (LEL) with one and two photon excitation of Quantum Dyes~R Lanthanide(III)-Macrocycles
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Lanthanide Enhanced Luminescence (LEL) with one and two photon excitation of Quantum Dyes~R Lanthanide(III)-Macrocycles

机译：量子染料〜R镧系元素（III）-大环的一和两个光子激发的镧系元素增强发光（LEL）

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摘要

Improvements in the lanthanide enhanced luminescence (LEL) protocol have facilitated the use of the recently synthesized Eu(III)-macrocycle-mono-isothiocyanate, Quantum Dye~R, as a label. It was discovered that a homogeneous solution in ethanol or other solvent could be used to produce the lanthanide enhanced luminescence (LEL) effect, provided that the solution was permitted to evaporate. This protocol has been applied to the direct staining of cells in S phase, and was optimized for solid phase assays with Quantum Dye labeled streptavidin. Preliminary studies indicate that cells stained with the europium Quantum Dye can be observed both by conventional UV laser excitation and by infrared two-photon confocal microscopy. An enhancer has been found that enables the observation of simultaneous emissions from both the europium and terbium Quantum Dyes.
机译：镧系元素增强发光（LEL）协议的改进促进了最近合成的Eu（III）-大环-单-异硫氰酸酯Quantum Dye_R作为标记的使用。发现在乙醇或其他溶剂中的均匀溶液可用于产生镧系元素增强的发光（LEL）效果，前提是该溶液可以蒸发。该方案已应用于S期细胞的直接染色，并针对用Quantum Dye标记的抗生蛋白链菌素进行固相分析进行了优化。初步研究表明，可以通过常规的紫外激光激发和红外双光子共聚焦显微镜观察到被Quan量子染料染色的细胞。已发现一种增强剂，可以观察of和ter量子染料的同时发射。

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