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首页> 外文会议>Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II; 20040127-20040128; San Jose,CA; US >Optical Tweezers as a Tool of Precision: Single-Molecule Mobility as Case Study
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Optical Tweezers as a Tool of Precision: Single-Molecule Mobility as Case Study

机译：镊子作为精密工具：以单分子移动为例

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摘要

We present a procedure for a precise power spectral analysis of optical tweezers data. This procedure uses the entire frequency range of the experimental power spectrum. We apply this procedure to experimental data from a biological system, data for the motion of a microsphere attached with a biotin-streptavidin linker to a single protein, the λ-receptor of E. coli. As in [Oddershede et al, Biophys. J. 83, 3152 (2002)] and [Oddershede et al, J. Phys.: Condens. Mat. 15, S1737 (2003)], we find that the λ-receptor moves diffusively in the outer membrane, but is confined to a particular region by a harmonic potential. Here, we show that since the power spectrum is known with precision up to its Nyquist frequency of 8 kHz, one can resolve the relative motion of the λ-receptor and the microsphere attached to it. We find that the biotin-streptavidin link between them can be described as a Hookean spring. Since we can see only the microsphere, these results are based on an interpretation of the power spectrum of its motion. This interpretation is based on a model. We use the simplest model that is necessary and sufficient to interpret the power spectrum in its full range. This model fits the spectrum well and the fit yields the model's parameters with some precision. These results indicate that optical tweezers have the potential to become a tool of precision. We discuss improvements of experiments and analysis that are necessary in order to realize this potential.
机译：我们提出了一种对光镊数据进行精确功率谱分析的程序。此过程使用了实验功率谱的整个频率范围。我们将此程序应用于来自生物系统的实验数据，即与生物素-链霉亲和素接头连接至单个蛋白质（大肠杆菌的λ受体）的微球运动的数据。如[Oddershede等人，Biophys。 J. 83，3152（2002）]和[Oddershede et al。，J. Phys .: Condens。垫。 15，S1737（2003）]，我们发现λ受体在外膜中扩散扩散，但受谐波电位限制在特定区域。在这里，我们表明，由于能以高达8 kHz的奈奎斯特频率精确地获知功率谱，因此可以解决λ受体和与其相连的微球的相对运动。我们发现，它们之间的生物素-链霉亲和素链接可以描述为胡木泉。因为我们只能看到微球，所以这些结果是基于对它运动的功率谱的解释。这种解释是基于模型的。我们使用必要和足够的最简单模型来解释其整个范围内的功率谱。该模型很好地拟合了光谱，并且拟合以一定的精度产生了模型的参数。这些结果表明，光镊有可能成为精密工具。我们讨论了改进实验和分析的必要条件，以实现这一潜力。

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