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首页> 外文会议>Conference on Laser Tissue Interaction XIII: Photochemical, Photothermal, and Photomechanical, Jan 20-23, 2002, San Jose, California >Microbubble dynamics around melanosomes irradiated with microsecond pulses
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Microbubble dynamics around melanosomes irradiated with microsecond pulses

机译：微秒脉冲辐照的黑素体周围的微泡动力学

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摘要

The origin of cell damage due to irradiation of the retina with microsecond laser pulses is most likely the disintegration of retinal pigment epithelial cells, which is caused by the formation of microbubbles around the strongly absorbing melanosomes within the cell. In order to get a more detailed understanding of the laser tissue interaction in the retinal pigment epithelium we irradiated a suspension of porcine melanosomes, which served as a model system, by a frequency doubled Nd:YLF laser emitting at 527nm. We used pulse durations of 500ns and 3.5μs. The formation of microbubbles around isolated melanosomes was imaged directly on a microscopic level by fast flash light photography. Near threshold radiant exposure for bubble formation we found transient bubbles with diameters below 1μm and lifetimes below 500ns. Applying super-threshold irradiation stable bubbles with diameters up to 7μm and lifetimes in the millisecond time regimen were observed. This can be explained by production of stable gas inside the melanosome due to laser heating.
机译：由于用微秒激光脉冲照射视网膜而引起的细胞损伤的起因很可能是视网膜色素上皮细胞的崩解，这是由于在细胞内强烈吸收的黑素体周围形成了微气泡而引起的。为了更详细地了解视网膜色素上皮中的激光组织相互作用，我们通过在527nm处发射的倍频Nd：YLF激光辐照了用作模型系统的猪黑素体悬浮液。我们使用了500ns和3.5μs的脉冲持续时间。通过快速闪光摄影直接在微观水平上对分离的黑素体周围的微气泡的形成进行了成像。在接近气泡形成的阈值辐射照射下，我们发现了直径小于1μm且寿命低于500ns的瞬态气泡。观察到超阈值辐照稳定气泡的直径最大为7μm，并在毫秒时间范围内发现了寿命。这可以通过激光加热在黑体内部产生稳定的气体来解释。

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