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首页> 外文会议>Conference on Lasers in Dentistry IX Jan 26-27, 2003 San Jose, California, USA >Assessment of Bone Repair following the use of Anorganic Bone Graft and Membrane associated or not to 830nm Laser Light
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Assessment of Bone Repair following the use of Anorganic Bone Graft and Membrane associated or not to 830nm Laser Light

机译：使用或不与830nm激光相关的无机骨移植物和膜后评估骨修复

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The aim of this study was to assess the effect of LLLT (λ830nm, Thera lase, DMC Equipamentos, Sao Carlos, SP, Brazil, 40mW, CW, spot size 0.60mm, 161/cm~2 per session) on the repair of bone defects on the femur of Wistar albinus rats which were grafted with anorganic bovine bone associated or not to bovine bone membrane. Five randomized groups were studied: Ⅰ (Control); Ⅱ (anorganic bovine bone); Ⅲ (anorganic bovine bone+ LLLT); Ⅳ (anorganic bovine bone + bovine bone membrane) and Ⅴ (anorganic bovine bone + bovine bone membrane + LLLT). The animals were irradiated at every 48h during 15 days, the first irradiation was performed immediately after the procedure. The animals were irradiated transcutaneuosly in four points around the defect. At each point a dose of 4J/cm~2 was given totaling 16J/cm~2 per session. The animals were sacrificed 15, 21 and 30 days after surgery. The specimens were routinely processed to wax and stained with H&E and Picrosirius stains and analyzed under light microscopy. The results showed evidence of a more advanced repair on the irradiated groups when compared to non-irradiated ones. The repair of irradiated groups was characterized by both increased bone formation and amount of collagen fibers around the graft within the cavity early, considering the osteoconductive capacity of the anorganic bovine bone and the increment of the cortical repair in specimens with membrane. It is concluded that LLLT had a positive effect on the repair of bone defect submitted the implantation of graft associated or not to the use of biological membrane.
机译：这项研究的目的是评估LLLT（λ830nm，Thera激光，DMC Equipamentos，Sao Carlos，SP，巴西，40mW，CW，光斑尺寸0.60mm，每节161 / cm〜2）对骨修复的影响Wistar albinus大鼠股骨上的缺损被移植了与或不与牛骨膜相关的无机牛骨。研究了五个随机分组：Ⅰ（对照组）； Ⅱ（无机牛骨）； Ⅲ（无机牛骨+ LLLT）； Ⅳ（无机牛骨+牛骨膜）和Ⅴ（无机牛骨+牛骨膜+ LLLT）。在15天内每48小时对动物进行一次辐照，手术后立即进行第一次辐照。在缺损周围的四个点处透皮照射动物。在每个点上，每次疗程给予4J / cm〜2的剂量，总计16J / cm〜2。在手术后15、21和30天处死动物。常规将样品加工成蜡，并用H＆E和Picrosirius染色，然后在光学显微镜下进行分析。结果表明，与未辐照组相比，辐照组的修复程度更高。考虑到无机牛骨的骨传导能力和带有膜的标本的皮质修复的增加，受辐照组的修复的特征是尽早增加了骨的形成，并增加了腔内移植物周围胶原纤维的数量。结论是，LLLT对骨缺损的修复具有积极作用，无论是否植入生物膜均与移植相关。

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