首页> 外文会议>Conference on Manipulation and Analysis of Biomolecules, Cells, and Tissues Jan 28-29, 2003 San Jose, California, USA >Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells
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Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells

机译:时间分辨光谱和显微镜下活细胞膜动力学的测量

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摘要

Fluorescence spectra and lifetime images of cultivated CHO cells incubated with the membrane marker laurdan are reported. The plasma membrane and intracellular membranes were distinguished using illumination of either the cell surface (by an evanescent wave) or of the whole cell. Parameters of membrane dynamics, probed by the generalized polarization and the effective fluorescence lifetime of laurdan, were correlated with temperature, age and growth phase of the cells.
机译:报告了与膜标记物laurdan一起孵育的培养CHO细胞的荧光光谱和寿命图像。通过照射细胞表面(通过e逝波)或整个细胞来区分质膜和细胞内膜。膜动力学的参数,通过广义偏振和劳登的有效荧光寿命来探测,与温度,年龄和细胞的生长阶段相关。

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