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首页> 外文会议>Conference on Manipulation and Analysis of Biomolecules, Cells, and Tissues Jan 28-29, 2003 San Jose, California, USA >AFM ANALYSIS OF THE EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) RELEASED DURING BACTERIAL ATTACHMENT ON POLYMERIC SURFACES
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AFM ANALYSIS OF THE EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) RELEASED DURING BACTERIAL ATTACHMENT ON POLYMERIC SURFACES

机译：细菌附着在聚合物表面上时释放的胞外聚合物（AFM）的AFM分析

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Extracellular polymeric substances (EPS) secreted by bacteria have a key role in adhesion and aggregation of bacterial cells on solid surfaces. In the present study, atomic force microscopy (AFM) has been used to study the adhesion propensity of bacterial strain St. guttiformis, and the ultrastructure and distribution of the EPS materials, on hydrophobic poly(tert-butylmethacrylate) (PtBMA) and hydrophilic polystyrene maleic acid (PSMA) surfaces. The results showed that bacterial attachment to the PSMA surface over incubation periods of 24-72 h was insignificant, whereas there was a strong propensity for the bacterial cells to attach to the PtBMA surface, forming multi-layered biofilms. For the PSMA surface, planktonic EPS adsorbed onto the polymeric surface and formed a continuous surface layer. For the PtBMA surface, non-contact mode imaging revealed that capsular EPS on the cell surface exhibited granular structures with the lateral dimensions of 30-50 nm and the vertical roughness of 7-10 nm. Lateral force imaging showed inter-connected elongated features which had lower frictional property compared to the surrounding EPS matrix, suggesting possible segregation of hydrophobic fractions of the EPS materials. The planktonic EPS adsorbed onto the PtBMA surface also showed similar nanometer-scale granular structures and could form stacks up to 150 nm in height. However, lateral force imaging did not show frictional differences, as in the case of capsular EPS. This is attributed to possible differences in the composition of the two EPS materials, and/or greater deformation of the planktonic EPS in the contact imaging mode which may obscure the fine surface features.
机译：细菌分泌的细胞外聚合物（EPS）在细菌细胞在固体表面的粘附和聚集中起关键作用。在本研究中，原子力显微镜（AFM）已用于研究细菌菌株Guttiformis的黏附倾向以及EPS材料在疏水性聚甲基丙烯酸叔丁酯（PtBMA）和亲水性聚苯乙烯上的超微结构和分布。马来酸（PSMA）表面。结果表明，细菌在24-72 h的孵育时间内附着于PSMA表面的作用不明显，而细菌细胞附着于PtBMA表面的趋势很强，形成了多层生物膜。对于PSMA表面，浮游EPS吸附到聚合物表面上并形成连续的表面层。对于PtBMA表面，非接触模式成像显示，细胞表面的荚膜EPS表现出颗粒结构，其横向尺寸为30-50 nm，垂直粗糙度为7-10 nm。横向力成像显示了相互连接的细长特征，与周围的EPS基质相比，它们具有较低的摩擦性能，表明EPS材料的疏水部分可能会分离。吸附在PtBMA表面上的浮游EPS也显示出相似的纳米级颗粒结构，并可能形成高达150 nm的堆栈。然而，横向力成像没有显示出摩擦差异，如囊EPS的情况。这归因于两种EPS材料的成分可能存在差异，和/或在接触成像模式中浮游EPS的较大变形可能会掩盖精细的表面特征。

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