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首页> 外文会议>Conference on Manipulation and Analysis of Biomolecules, Cells, and Tissues Jan 28-29, 2003 San Jose, California, USA >Response of living cells to nano-structured polyelectrolyte matrices studied by means of 1-, 2- photon excitation microscopy
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Response of living cells to nano-structured polyelectrolyte matrices studied by means of 1-, 2- photon excitation microscopy

机译：通过1、2-光子激发显微镜研究活细胞对纳米结构聚电解质基质的响应

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摘要

Three-dimensional confocal laser scanning microscopy (CLSM) and two-photon excitation microscopy (TPEM) were used to study the response of cellular systems to fuzzy organized nanostructured polyelectrolytes used both as microcontainers and microcarriers for drug delivery. These nanostructured systems are named Nanocapsules and represent a new class of controllable colloids. CLSM and TPEM uniquely allow to follow the fate of encapsulated living cells and to track the pathway of nanocapsules introduced into cellular systems. For the former situation, it will be shown how living cells can be encapsulated and demonstrated the preservation of the metabolic and duplicating activity. In this case the role of the Nanocapsule is as microcontainer endowed of functionalized surface and of protective ability. The latter situation, is related to feeding living cells with Nanocapsules. This experiment serves in elucidating the comprehension of the potential cytotoxicity and of the ability of Nanocapsules to reach specific targets where active compounds can be released. Cellular systems used within this research are Saccharomyces cerevisiae and Paramecium primaurelia living cells. In the case of encapsulation of Saccharomyces cerevisiae living cells, the most relevant result is that, after encapsulation, cells preserve their metabolic activities and they are still able to divide. At this stage is also relevant the utilization of spectroscopic methods like fluorescence lifetime and second harmonic imaging. These hybrid polyelectrolyte-cells can provide a cheap model system in a wide range of biophysical and biotechnological applications, thanks to the tunable properties of the polyelectrolyte shell.
机译：使用三维共聚焦激光扫描显微镜（CLSM）和双光子激发显微镜（TPEM）来研究细胞系统对用作药物微容器和微载体的模糊组织的纳米结构聚电解质的响应。这些纳米结构的系统称为纳米胶囊，代表了一类新的可控胶体。 CLSM和TPEM独特地允许遵循封装的活细胞的命运，并追踪引入细胞系统的纳米胶囊的路径。对于前一种情况，将展示如何封装活细胞，并展示了新陈代谢和复制活性的保存。在这种情况下，纳米胶囊的作用是作为具有功能化表面和保护能力的微容器。后一种情况与用纳米胶囊喂养活细胞有关。该实验用于阐明对潜在细胞毒性的理解以及纳米胶囊达到可以释放活性化合物的特定靶标的能力。本研究中使用的细胞系统是酿酒酵母和原草履虫草活细胞。在封装酿酒酵母活细胞的情况下，最相关的结果是，在封装后，细胞保留了其代谢活性，并且仍然能够分裂。在这一阶段，与光谱方法如荧光寿命和二次谐波成像的利用也相关。由于聚电解质壳的可调性，这些杂化的聚电解质电池可以在广泛的生物物理和生物技术应用中提供便宜的模型系统。

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