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Imaging single metal nanoparticles in cells by photothermal interference contrast

机译:通过光热干涉对比成像细胞中的单个金属纳米粒子

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摘要

We have developed a photothermal method for far-field optical detection of nanometer-sized metal particles, combining high-frequency modulation and polarization interference contrast. We can image gold colloids down to 5 nm in diameter, with a signal-to-noise ratio higher than 10. This is a considerable improvement over commonly used optical methods based on resonance plasmon scattering which, for background reasons, are limited to particles of more than about 40 nm in diameter. We also show that in addition to its intrinsic sensitivity, our photothermal method is totally insensitive to non-absorbing scatterers as 10 nm nanoparticles can be imaged in cells.
机译:我们开发了一种光热方法,结合了高频调制和偏振干涉对比技术,可以对纳米级金属颗粒进行远场光学检测。我们可以对直径小于5 nm的金胶体成像,信噪比大于10。与基于共振等离子体激元散射的常用光学方法相比,这是一个很大的改进,由于背景原因,该方法仅限于以下粒子直径大于约40 nm。我们还表明,除了其固有的灵敏度外,我们的光热方法对非吸收性散射体完全不敏感,因为可以在细胞中成像10 nm纳米颗粒。

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