• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Microfluidics, BioMEMS, and Medical Microsystems, Jan 27-29, 2003, San Jose, California, USA >Single Cell Analysis on a Microchip Platform using Optical Tweezers and Optical Scissors
【24h】

Single Cell Analysis on a Microchip Platform using Optical Tweezers and Optical Scissors

机译：使用光镊和光剪刀在Microchip平台上进行单细胞分析

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microfluidic devices, or "lab-on-a-chip" systems for single cell analysis represent a new field of micro-total analysis systems (μTAS) that could not only perform a task quicker, and more accurately than conventional methods, but could also incorporate additional tools to the study of biological variability in a population by allowing researchers to directly examine the contents of a multitude of single cells from the population under study. Realizing such a device, presents several engineering challenges to the fields of micro-machining, micromanipulation and analytical bio-chemistry. The device needs to be able to accurately and efficiently select, manipulate and analyze volumes represented by a single cell without diluting the contents. For this purpose, optical tweezers and scissors were implemented to select single cells on a microchip, bring the cell to a desired location, and lyse the cell using the optical scissors. Channels were engineered in the device using a molecular fluorine (F_2) laser. Each channel's cross-section is approximately the size of an individual cell (10μm wide and deep). This paper, describes the manipulation of cells on a microchip using optical tweezers and the injection of the cellular contents by optical scissors from a single cell into 10μm channels.
机译：微流体设备或用于单细胞分析的“芯片实验室”系统代表了微总分析系统（μTAS）的新领域，它不仅比传统方法更快，更准确地执行任务，而且还可以通过允许研究人员直接检查被研究种群中大量单个细胞的含量，将其他工具整合到研究种群中的生物变异性中。实现这种装置，对微加工，微处理和分析生物化学领域提出了一些工程挑战。该设备需要能够在不稀释内容物的情况下准确，高效地选择，操作和分析单个单元格代表的体积。为此目的，实施了光镊和剪刀以选择微芯片上的单个细胞，将细胞移至所需位置，并使用光剪刀裂解细胞。使用分子氟（F_2）激光在设备中设计通道。每个通道的横截面大约是单个单元的大小（宽10μm，深）。本文介绍了使用光镊对微芯片上的细胞进行处理，以及用光剪刀将细胞内的物质从单个细胞注入10μm通道的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号