• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Molecular Probes for Biomedical Applications; 20080121-22; San Jose,CA(US) >Development of Fluorescent Contrast Agents for Optical Imaging of Mouse Disease Models
【24h】

Development of Fluorescent Contrast Agents for Optical Imaging of Mouse Disease Models

机译：用于小鼠疾病模型光学成像的荧光造影剂的开发

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Optical imaging is a rapidly developing field of research aimed at non-invasively interrogating animals for disease progression, determining the effects of a drug on a particular pathology, assessing the pharmacokinetic behavior of a drug, or identifying molecular biomarkers of disease. One of the key components of molecular imaging is the development of specific, targeted imaging contrast agents to assess these biological processes. The development of robust fiuorochrome-labeled optical agents is a process that is often underestimated in terms of its complexity. We describe here the development process and performance issues for three different optical agents: IRDye 800CW EGF (epidermal growth factor), IRDye~R 800CW 2-DG (2-deoxy D-glucose), and an IRDye 680 BoneTag~(TM). In vitro competitive assays were developed for two of the markers to demonstrate specificity. Specificity was confirmed in animal studies. Uptake of IRDye 800CW 2-DG was also examined by near-infrared confocal microscopy. Histological examinations were performed on target and non-target tissues following the completion of the imaging studies. The issues unique to the development of each labeled marker are discussed.
机译：光学成像是快速发展的研究领域，旨在以无创方式询问动物的疾病进展，确定药物对特定病理的影响，评估药物的药代动力学行为或鉴定疾病的分子生物标记。分子成像的关键组成部分之一是开发特定的靶向成像造影剂以评估这些生物学过程。耐用的荧光染料标记的光学试剂的开发是一个经常因其复杂性而被低估的过程。我们在此描述三种不同光学试剂的开发过程和性能问题：IRDye 800CW EGF（表皮生长因子），IRDye〜R 800CW 2-DG（2-脱氧D-葡萄糖）和IRDye 680 BoneTagTM。针对两种标记物进行了体外竞争性测定，以证明其特异性。在动物研究中证实了特异性。还通过近红外共聚焦显微镜检查了IRDye 800CW 2-DG的吸收。影像学研究完成后，对目标和非目标组织进行组织学检查。讨论了每个标记标记的开发所特有的问题。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号