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首页> 外文会议>Conference on Multiphoton Microscopy in the Biomedical Sciences IV; 20040125-20040127; San Jose,CA; US >Use of Optical Parametric Oscillator in Scanning Optical Microscopy
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Use of Optical Parametric Oscillator in Scanning Optical Microscopy

机译：光学参量振荡器在扫描光学显微镜中的使用

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Ultrafast lasers have found increasing use in scanning optical microscopy due to their very high peak power in generating multiphoton excitations. A mode-locked Ti:sapphire laser is often employed for such purposes. Together with a synchronously pumped optical parametric oscillator (OPO), the spectral range available can be extended to 1050-1300 nm. This broader range available greatly facilitates the excitation of second harmonic generation (SHG) and third harmonic generation (THG) due to better satisfaction of phase matching condition that is achieved with a longer excitation wavelength. Dental sections are then investigated with the contrasts from harmonic generation. In addition, through intra-cavity doubling wavelengths from 525-650 nm are made available for effective two-photon (2-p) excitation with the equivalent photon energy in the UVB range (290-320 nm) and beyond. This new capacity allows UV (auto-) fluorescence excitation and imaging, for example, from some amino acids, such as tyrosine, tryptophan, and glycine.
机译：由于超快激光器在产生多光子激发中具有非常高的峰值功率，因此已在扫描光学显微镜中得到越来越多的使用。为此，通常使用锁模的钛蓝宝石激光器。与同步泵浦光学参量振荡器（OPO）一起，可用光谱范围可以扩展到1050-1300 nm。由于可以用更长的激发波长来更好地满足相位匹配条件，因此这种更宽的可用范围极大地促进了二次谐波产生（SHG）和三次谐波产生（THG）的激发。然后用谐波产生的对比研究牙齿的断面。此外，通过腔内加倍波长，从525-650 nm可获得有效的两光子（2-p）激发，并具有UVB范围（290-320 nm）或更高的等效光子能量。这种新功能可以进行UV（自动）荧光激发和成像，例如，从某些氨基酸（例如酪氨酸，色氨酸和甘氨酸）中进行激发和成像。

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