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Fluorescence lifetime imaging with a multifocal two photon microscope

机译:多焦点两光子显微镜的荧光寿命成像

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摘要

Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long acquisition time for fluorescence lifetime imaging. Thus it is commonly limited to intensity images that only give indications on location of fluorophores but hardly reports physico-chemical properties and interactions between cells components. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while keeping the possibility to measure both intensity and lifetime images for all multifocal points.
机译:两光子显微镜是用于细胞或组织成像的强大工具。然而,它存在作为激光扫描技术的缺点,该技术导致荧光寿命成像的获取时间长。因此,通常仅限于强度图像,强度图像仅给出荧光团的位置指示,而几乎不报告理化性质和细胞成分之间的相互作用。为了保护过长的实验中的生物样本并提供更完整的光谱工具,我们开发了时间分辨多焦点多光子显微镜。这种设置使我们可以加快采集速度,同时保留测量所有多焦点强度和寿命图像的可能性。

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