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首页> 外文会议>Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA >Preparation and characterization of phantom objects for optical imaging by time-resolved transmittance and fluorescence
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Preparation and characterization of phantom objects for optical imaging by time-resolved transmittance and fluorescence

机译：时间分辨透射率和荧光的光学成像幻象物体的制备和表征

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摘要

Well-characterized phantom objects are necessary for investigating the performances of optical imaging systems based on time-resolved transmittance and fluorescence. For this purposes we have prepared inhomogeneous phantoms made of gelatinous objects placed in aqueous solutions of 10% Intralipid with different concentrations. The gelatinous objects have been prepared using a mixture of 10% Intralipid with agar at which absorbing ink have been added for transmittance based optical imaging system. For fluorescence measurements proper fluorescent dyes (rhodamine6G and IR125) have been added. Conventional optical characterization by spectrophotometric and spectrofluorimetric measurements have been performed. In addition, time-resolved transmittance and fluorescence measurements have been carried out. In particular, time-correlated single photon counting system has been used for time-resolved transmittance measurements. For time-resolved fluorescence measurements an optical imaging system based on a Ti:Sa laser and streak camera has been employed.
机译：表征良好的幻影对象对于研究基于时间分辨的透射率和荧光的光学成像系统的性能非常必要。为此，我们准备了由凝胶状物体制成的不均匀体模，该体模放置在浓度不同的10％脂质体内的水溶液中。凝胶状物体是使用10％脂质体和琼脂的混合物制备的，在该混合物中添加了吸收性油墨，用于基于透射率的光学成像系统。为了进行荧光测量，添加了适当的荧光染料（若丹明6G和IR125）。已经通过分光光度法和荧光分光光度法进行了常规的光学表征。另外，已经进行了时间分辨的透射率和荧光测量。特别是，时间相关的单光子计数系统已用于时间分辨的透射率测量。对于时间分辨的荧光测量，已使用基于Ti：Sa激光和条纹相机的光学成像系统。

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