Flow cytometric monitoring of hormone receptor expression in human solid tumors

机译：流式细胞仪监测人实体瘤中激素受体的表达

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Hormone receptor expression in human breast and prostate tumors is of diagnostic and therapeutic importance. With the availability of anti-estrogen, androgen and progesterone antibodies, immunohistochemistry has become a standard tool for determination of receptor expression in human tumor biopsies. However, this method is dependent on examination of a small number of cells under a microscope and the data obtained in most cases is not quantitative. As most of the commercially used anti-hormone antibodies have nuclear specificity, we have developed methods for isolation and antigen unmasking of nuclei from formalin fixed/paraffin embedded archival human tumors. After immunostaining with the antibodies and propidium iodide (for DNA content and cell cycle analysis), nuclei are analyzed by multiparametric laser flow cytometry for hormone receptor expression, DNA content, aneuploidy and cell cycle determination. These multiparametric methods are especially important for retrospective studies seeking to correlate hormone receptor expression with clinical response to anti-hormonal therapy of human breast and prostate tumors.

机译：人乳腺和前列腺肿瘤中激素受体的表达具有诊断和治疗意义。随着抗雌激素，雄激素和孕激素抗体的普及，免疫组织化学已成为确定人类肿瘤活检组织中受体表达的标准工具。然而，该方法取决于在显微镜下检查少量细胞，并且在大多数情况下获得的数据不是定量的。由于大多数市售的抗激素抗体具有核特异性，因此我们开发了从福尔马林固定/石蜡包埋的存档人肿瘤中分离和分离抗原核的方法。用抗体和碘化丙锭免疫染色后（用于DNA含量和细胞周期分析），通过多参数激光流式细胞术分析细胞核，以测定激素受体的表达，DNA含量，非整倍性和细胞周期测定。这些多参数方法对于寻求将激素受体表达与对人乳腺和前列腺肿瘤的抗激素治疗的临床反应相关的回顾性研究尤其重要。

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来源
《Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA》|2002年|p.211-217|共7页
会议地点 San Jose CA(US)
作者
Awtar Krishan;
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作者单位

Experimental Therapeutics Division (R-71), University of Miami Medical School, P.O. Box 01690, Miami, F1.USA;

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原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
estrogen; androgen; progesterone; hormone receptors; flow cytometry;

机译：雌激素雄激素黄体酮激素受体；流式细胞仪;

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专利

1. Flow cytometric analysis of estrogen, progesterone receptor expression and DNA content in formalin-fixed, paraffin-embedded human breast tumors. [J] . Redkar AA, Krishan A Cytometry: The Journal of the Society for Analytical Cytology . 1999,第2期

机译：流式细胞仪分析福尔马林固定，石蜡包埋的人乳腺肿瘤中的雌激素，孕激素受体表达和DNA含量。
2. Expression of mRNA for growth hormone-releasing hormone and splice variants of GHRH receptors in human malignant bone tumors. [J] . Busto R, Schally A, Braczkowski R, Regulatory peptides. . 2002,第2a3期

机译：人类恶性骨肿瘤中生长激素释放激素和GHRH受体剪接变体的mRNA表达。
3. Quantitative Immunoflporescent Estimation of Estrogen Receptor p Expression in Human Solid Tumors Using Flow Cytometry [J] . T. A. Bogush, A. S. Shaturova, E. A. Dudko Moscow University Chemistry Bulletin . 2011,第4期

机译：流式细胞术定量免疫估计人体实体肿瘤中雌激素受体p的表达
4. Expression Study of Estrogen Receptor-related Receptors and Steroid Hormone Receptors in Human Prostatic Cells [C] . Franky L. Chan, C.P. Cheung, Lung-Wai Chan, International Symposium on Hormonal Carcinogenesis . 2005

机译：雌激素受体相关受体和类固醇激素受体在人前列腺细胞中的表达研究
5. Transcription regulation of human gonadotropin-releasing hormone receptor gene expression. [D] . Cheng, Kwai Wa. 2001

机译：人类促性腺激素释放激素受体基因表达的转录调控。
6. Expression of tumour necrosis factor receptor and Toll-like receptor 2 and 4 on peripheral blood leucocytes of human volunteers after endotoxin challenge: a comparison of flow cytometric light scatter and immunofluorescence gating [O] . X Wittebole, S M Coyle, A Kumar, 2005

机译：内毒素攻击后人类志愿者外周血白细胞上肿瘤坏死因子受体及Toll样受体2和4的表达：流式细胞术光散射和免疫荧光门控的比较
7. Expression of tumour necrosis factor receptor and Toll-like receptor 2 and 4 on peripheral blood leucocytes of human volunteers after endotoxin challenge: a comparison of flow cytometric light scatter and immunofluorescence gating [O] . Wittebole, X, Coyle, S M, Kumar, A, 2005

机译：内毒素攻击后人类志愿者外周血白细胞上肿瘤坏死因子受体和Toll样受体2和4的表达：流式细胞仪光散射和免疫荧光门控的比较
8. IL-1 and Tumor Necrosis Factor-Alpha Each Up-Regulate Both the Expression of IFN-Gamma Receptors and Enhance IFN-Gamma-Induced HLA-DR Expression on Human Monocytes and a Human Monocytic Cell Line (THP-1) [R] . Krakauer, T., Oppenheim, J. J. 1993

机译：IL-1和肿瘤坏死因子-α各自上调IFN-γ受体的表达并增强人单核细胞和人单核细胞系（THp-1）上IFN-γ诱导的HLa-DR表达

Flow cytometric monitoring of hormone receptor expression in human solid tumors

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