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首页> 外文会议>Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA >Delayed Luminescence Technique: A Probe For Vital Processes In Biological Systems
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Delayed Luminescence Technique: A Probe For Vital Processes In Biological Systems

机译：延迟发光技术：生物系统中重要过程的探针。

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摘要

Delayed Luminescence is a well established technique based on the illumination of a biological sample and on the subsequent count of the number of photons re-emitted by the sample after the light source has been switched off together with their spectral distribution. Investigations have been performed on yeast cells and algae so that correlations can be established between biological activities and physical parameters of the samples. Moreover nonlinear mechanisms of interaction between optical fields and cells can be figured out. Further investigations will be reported on yeast cell samples deposited on paper filters after irradiation by soft X-rays. The results will be discussed by cross correlating the experimental evidence from Delayed Luminescence with those obtained by metabolic activity recording. Luminescence and Delayed Luminescence are strictly correlated with early insurgence of morphological alterations of normal or pathological nature in cells and tissues; a novel technique for morphological analysis has been developed by means of Focused Ion Beam machines. A straightforward approach to morphology at the nanoscale both of membranes and cellular inner structures is then made possible. The final aim is an experimental set up for an early and reliable detection technique for neoplastic cells and tissues sorting.
机译：延迟发光是一种成熟的技术，其基于生物样品的照明以及在光源关闭后，样品重新发射的光子数量及其光谱分布的后续计数。已经对酵母细胞和藻类进行了研究，以便可以在样品的生物学活性和物理参数之间建立相关性。此外，可以找出光场与细胞之间相互作用的非线性机理。对于软X射线照射后沉积在纸滤器上的酵母细胞样品，将进行进一步研究。将通过将延迟发光的实验证据与通过代谢活性记录获得的证据进行互相关来讨论结果。发光和延迟发光与细胞和组织中正常或病理性形态改变的早期抑制密切相关。通过聚焦离子束机已经开发出一种新的形态分析技术。这样就可以在膜和细胞内部结构的纳米级上直接采用形态学方法。最终目标是为肿瘤细胞和组织分类的早期可靠检测技术建立实验装置。

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