首页> 外文会议>Conference on Optical Diagnostics and Sensing IV; 20040127; San Jose,CA; US >Detection of integrins using surface enhanced Raman Spectroscopy
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Detection of integrins using surface enhanced Raman Spectroscopy

机译:使用表面增强拉曼光谱检测整联蛋白

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Integrins play an important role in the adhesion of cells to extracellular matrix and to other cells around them, more specifically fibronectin. The ultimate goal of this research is to detect these integrins on the surface of the cell with a combined atomic force microscopy (AFM) system coupled with a surface enhanced Raman spectroscopy (SERS) system. For this paper the focus was on identifying whether SERS is capable of being used to generate a unique spectrum for integrins. This was done using silver colloidal particles and the integrins a5pl and aVp3. It was shown that a unique spectrum could be identified for each of these integrins at the nanomolar level.
机译:整联蛋白在细胞粘附到细胞外基质和细胞周围其他细胞,特别是纤连蛋白上起重要作用。这项研究的最终目的是通过结合原子力显微镜(AFM)系统和表面增强拉曼光谱(SERS)系统检测细胞表面的这些整合素。在本文中,重点是确定SERS是否能够用于生成整联蛋白的独特光谱。使用银胶体颗粒和整联蛋白a5p1和aVp3来完成。结果表明,可以在纳摩尔水平上为每种整合素鉴定出独特的光谱。

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