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首页> 外文会议>Conference on Optical Diagnostics and Sensing IV; 20040127; San Jose,CA; US >Raman spectroscopy and Raman imaging for early detection of cancer
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Raman spectroscopy and Raman imaging for early detection of cancer

机译：拉曼光谱和拉曼成像可早期发现癌症

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Raman spectroscopy is a powerful technique as it provides fundamental information about vibrational modes of a system. Eigenvalues of these modes are very sensitive to the strength of the chemical bonds and perturbations caused by the environment, particularly charge distribution and alterations in the dipole strength of the system. All these parameters are profoundly modified during the tumor formation process and hence Raman technique could be a unique and also a direct approach for evaluating tumor genesis at early stages. For this purpose the present investigation was carried out. We used cultured wild type and c-ras transformed NIH 3T3 fibroblast. The samples were treated with methyl alcohol solution in a conventional manner and then Raman spectra and images were obtained by a specially developed confocal Raman microscope. The present results reveal differences between both cell types in the spectral details as well as in the morphology. Raman images are able to detect the exact site where cancer-related changes have taken place. These results clearly indicate the superiority of the present technique over conventional methods such as images obtained by X -rays or Nuclear Magnetic Resonance (NMR). Moreover, unlike other approaches, Raman images detect alterations at the submicron level rather than in the centimeter or millimeter range. Being an optical method it can be applied in vivo as a non-invasive technique potentially useful to early detection of cancer (particularly easy accessible cancers such as those of the skin and the digestive tract). The obtained results suggest the great potential of Raman imaging in premature clinical diagnostic approaches.
机译：拉曼光谱法是一项强大的技术，因为它提供了有关系统振动模式的基本信息。这些模式的特征值对环境引起的化学键和扰动的强度非常敏感，特别是电荷分布和系统偶极强度的变化。所有这些参数在肿瘤形成过程中都得到了深刻的修改，因此拉曼技术可能是早期评估肿瘤发生的独特且直接的方法。为此，进行了本研究。我们使用培养的野生型和c-ras转化的NIH 3T3成纤维细胞。以常规方式用甲醇溶液处理样品，然后通过专门开发的共聚焦拉曼显微镜获得拉曼光谱和图像。目前的结果揭示了两种细胞类型之间在光谱细节以及形态上的差异。拉曼图像能够检测出与癌症有关的变化的确切部位。这些结果清楚地表明，本技术相对于常规方法，例如通过X射线或核磁共振（NMR）获得的图像的优越性。此外，与其他方法不同，拉曼图像可检测到亚微米级别的变化，而不是厘米或毫米范围内的变化。作为一种光学方法，它可以作为一种非侵入性技术在体内应用，可能对癌症的早期检测很有用（特别是容易获得的癌症，例如皮肤和消化道的癌症）。获得的结果表明，拉曼成像在过早的临床诊断方法中具有巨大的潜力。

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