The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe, cultured at 37℃

机译：流式细胞仪在37℃培养血清素对鼠疫微生物抗原组成和DNA含量的作用中的应用

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The results have been obtained by flow cytometry showed the significant raise of fluorescence intensity of Yersinia pestis EV cells binding FITC-labelled plague polyclonal immunoglobulins after 24 h cultivation in Hottinger broth pH 7.2 at 37℃ in the presence of increasing concentration serotonin (5-Hydroxytryptamine). The presence of serotonin (5-HT) in nutrient broth at concentration of 10~(-5) M, 10~(-8) M could modulate DNA content in 37 ℃ growing population of plague microbe. The effect of this action depended of 5-HT concentration.

机译：流式细胞仪检测结果表明，在浓度增加的5-羟色胺（5-羟色胺）存在下，于37℃在Hottinger肉汤pH 7.2中培养24 h后，结合FITC标记的鼠疫多克隆免疫球蛋白的鼠疫耶尔森氏菌EV细胞的荧光强度显着提高。）。营养液中5-羟色胺（5-HT）浓度为10〜（-5）M，10〜（-8）M时，可以调节37℃鼠疫微生物种群中的DNA含量。该作用的效果取决于5-HT的浓度。

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《Conference on Optical Technologies in Biophysics and Medicine IV; Oct 1-4, 2002; Saratov, Russia》|2002年|p.61-67|共7页
会议地点 Saratov(RU);Saratov(RU);Saratov(RU);Saratov(RU);Saratov(RU)
作者
Vladimir N.Korsukov; Tatyana N.Schukovskaya; Svetlana N.Klueva; Alexander L.Kravtsov; Tatyana A.Polunina; Youri A.Popov;
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作者单位

Russian Anti-Plague Research Institute "Microbe"of Ministry of Health of Russian Federation Universitetskaya 46, 410005, Saratov, Russia;

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会议组织
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
flow cytometry; yersinia pestis; serotonin influence; binding of FITC-labeled antibodies; DNA content;

机译：流式细胞仪;鼠疫耶尔森菌; 5-羟色胺影响; FITC标记抗体的结合; DNA含量;

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1. Primary characterization of the properties and diagnostic value of the antigenic complex "fraction V" in a plague microbe [J] . Bozhko NV, Lebedeva SA, Bichul OK, Клиничесκая лабораторная диагностиκа . 2005,第6期

机译：鼠疫微生物中抗原复合物“馏分V”的性质和诊断价值的初步表征
2. Primary characterization of the properties and diagnostic value of the antigenic complex "fraction V" in a plague microbe [J] . Bozhko NV, Lebedeva SA, Bichul OK, Клиничесκая лабораторная диагностиκа . 2005,第6期

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3. Combined flow cytometry determination of S-phase fraction and DNA ploidy is an independent prognostic factor in node-negative invasive breast carcinoma: review of a series of 271 patients with stage I and II breast cancer [J] . Moureau Zabotto L, Bouchet C, Cesari D, Cancer radiotherapie: journal de la Soci閠?fran鏰ise de radiotherapie oncologique . 2005,第8期

机译：联合流式细胞术测定S期分数和DNA倍性是淋巴结阴性浸润性乳腺癌的独立预后因素：对271例I，II期乳腺癌患者的回顾
4. The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe, cultured at 37°C [C] . Vladimir N.Korsukov, Tatyana N.Schukovskaya, Svetlana N.Klueva, Annual international multidisciplinary school for young scientists and students on optics, laser physics and biophysics . 2003

机译：流式细胞术的应用血清素抗原组合物法测定瘟疫微生物的抗原组合物的作用，37℃培养培养
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The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe, cultured at 37℃

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