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首页> 外文会议>Conference on Optical Technologies in Biophysics and Medicine IV; Oct 1-4, 2002; Saratov, Russia >FUNCTIONAL SPECTROSCOPY APPROACH TO THE ASSESSMENT OF NITRIC OXIDE STORAGE IN VASCULAR TISSUES
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FUNCTIONAL SPECTROSCOPY APPROACH TO THE ASSESSMENT OF NITRIC OXIDE STORAGE IN VASCULAR TISSUES

机译：血管组织中一氧化二氮储存量的功能光谱法

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Much attention has been devoted to the enzymatic production of nitric oxide (NO) by the endothelial layer lining blood vessel walls, which regulates among other things local vasodilatation and platelet adhesion. Considerably less attention, however, has been paid to the accumulation of NO-related products in the vascular wall itself. Such local storage of NO products could conceivably contribute to the local regulation of blood flow and provide additional anti-adhesive protection, if biochemically activated to regenerate NO. Since little is known about their chemical nature, concentrations, and possible role in vascular biology we sought to characterize those species basally present in rat aorta. To this end we developed a functional form of optical spectroscopy that allows us not only to identify NO-stores in intact tissues but also to monitor their production and disappearance in real-time. The method is based on the ability of NO stores to reversibly release NO when illuminated with light of particular wavelengths, which can be detected as a robust relaxation of vascular smooth muscle (photorelaxation). Characterization of NO-stores is achieved through a careful assessment of photorelaxation action spectra, taking into account the light scattering properties of the tissue, and of depletion of the NO-stores induced by exposure to controlled levels of light. This functional form of optical spectroscopy is applied to rat aortic tissue where the results suggest that the NO photolytically released from tissue stores originated from a low-molecular-weight RSNO as well as from nitrite. The significance of these findings to vascular physiology and pathophysiology is discussed.
机译：通过血管壁衬里的内皮层，一氧化氮（NO）的酶促产生已引起人们的广泛关注，该调节除其他因素外还调节局部血管舒张和血小板粘附。然而，已经很少关注血管壁自身中NO相关产物的积累。如果生化激活NO的再生，则此类NO产品的局部存储可能有助于局部调节血流并提供额外的抗粘连保护。由于对其化学性质，浓度和在血管生物学中的可能作用了解甚少，我们试图表征大鼠主动脉中基本存在的那些物种。为此，我们开发了一种功能形式的光谱学，不仅使我们能够识别完整组织中的NO储存，而且能够实时监测其产生和消失。该方法基于NO储存器在被特定波长的光照射时可逆释放NO的能力，可以将其检测为血管平滑肌的强烈松弛（光松弛）。通过仔细评估光松弛作用谱来实现NO储存的表征，同时考虑到组织的光散射特性以及暴露于受控光水平引起的NO储存的耗尽。这种光谱形式的功能形式适用于大鼠主动脉组织，结果表明大鼠组织存储中光解性释放的NO源自低分子量RSNO以及亚硝酸盐。讨论了这些发现对血管生理和病理生理的意义。

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