Tissue morphology from spectral polarimetry

机译：光谱极化仪的组织形态

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An important issue in the development of cultured tissues is the alignment of the cells within the scaffold, or on the substrate. Proper alignment leads to optimum tissue strength and it has been demonstrated that proper alignment is engendered by application of physiologically realistic stresses during the cell proliferation process. In situ monitoring of cell alignment during development can provide important feedback information in determining the optimum stresses. Numerical calculations suggest that cell aspect and orientation can be inferred from the polarization of the light scattered by these cells. In this paper, we demonstrate that a measurement of the wavelength-dependent depolarization of the light scattered from the cell layer reveals the alignment of these cells. We present results of experimental measurements on human umbilical vein endothelial cells (HUVEC's) layered onto glass cover slips and of simulations using T-matrix methods.

机译：培养的组织发育中的重要问题是支架内或基质上细胞的排列。正确的对齐会导致最佳的组织强度，并且已经证明在细胞增殖过程中通过施加生理上的实际应力可以实现正确的对齐。在发育过程中对细胞排列的原位监测可以为确定最佳应激提供重要的反馈信息。数值计算表明，可以从这些细胞散射的光的偏振中推断出细胞的长宽比和方向。在本文中，我们证明了对从细胞层散射的光的波长依赖性去极化的测量揭示了这些细胞的排列。我们介绍了对人脐静脉内皮细胞（HUVEC）分层到玻璃盖玻片上的实验测量结果，以及使用T-矩阵方法进行模拟的结果。

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来源
《Conference on Optics in Tissue Engineering and Regenerative Medicine; 20080120-21; San Jose,CA(US)》|2008年|P.iviii|共2页
会议地点 San JoseCA(US)
作者
Donald D. Duncan; James C. Gladdish; Brandon D. Markway;
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作者单位

Oregon Health Science University, 3303 SW Bond Ave, Portland, OR USA 97239-4501;

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会议组织
原文格式 PDF
正文语种 eng
中图分类医用物理学;
关键词
polarization; structure; spectral;

机译：极化;结构;光谱;

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机译：组织芯片上乳腺癌的光谱分析：超越形态学。

Tissue morphology from spectral polarimetry

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