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首页> 外文会议>Conference on Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 20080121-22; San Jose,CA(US) >Noninvasive Imaging In Vivo With Fluorescent Proteins From Centimeters To Micrometers
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Noninvasive Imaging In Vivo With Fluorescent Proteins From Centimeters To Micrometers

机译：从厘米到微米的荧光蛋白体内无创成像。

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Whole-body imaging with fluorescent proteins has been shown to be a powerful technology with many applications in small animals. Our laboratory pioneered in vivo imaging with fluorescent proteins (1) including noninvasive whole-body imaging (2). Whole-body imaging with fluorescent proteins depends in large part on the brightness of the protein. Brighter, red-shifted proteins can make whole-body imaging more sensitive due to reduced absorption by tissues and less scatter. Non-invasive imaging with fluorescent proteins has been shown to be able to quantitatively track tumor growth and metastasis, gene expression, angiogenesis, and bacterial infection (3) even at subcellular resolution depending on the position of the cells in the animal. Interference by skin autofluorescence is kept to a minimum with the use of proper filters. To noninvasively image cancer cell/stromal cell interaction in the tumor microenvironment and drug response at the cellular level in live animals in real time, we developed a new imageable three-color animal model. The model consists of green fluorescent protein (GFP)-expressing mice transplanted with dual-color cancer cells labeled with GFP in the nucleus and red fluorescent protein (RFP) in the cytoplasm. Various in vivo phenomena of tumor-host interaction and cellular dynamics were imaged, including mitotic and apoptotic tumor cells, stromal cells interacting with the tumor cells, tumor vasculature, and tumor blood flow as well as drug response. This imageable technology should lead to many new insights of in vivo cancer cell biology.
机译：荧光蛋白的全身成像已被证明是一项强大的技术，在小型动物中有许多应用。我们的实验室率先使用荧光蛋白进行体内成像（1），包括无创全身成像（2）。用荧光蛋白进行的全身成像在很大程度上取决于蛋白的亮度。由于组织吸收的减少和散射的减少，较亮的红移蛋白可以使全身成像更加敏感。荧光蛋白的非侵入性成像已被证明能够定量追踪肿瘤的生长和转移，基因表达，血管生成和细菌感染（3），即使在亚细胞分辨率下也取决于动物细胞的位置。使用适当的过滤器，可使皮肤自发荧光的干扰减至最小。为了在活体动物中实时对肿瘤微环境中的癌细胞/基质细胞相互作用和细胞水平的药物反应进行无创成像，我们开发了一种新的可成像三色动物模型。该模型由表达绿色荧光蛋白（GFP）的小鼠组成，这些小鼠移植有在细胞核中标记有GFP的双色癌细胞和在细胞质中标记有红色荧光蛋白（RFP）的小鼠。对体内各种肿瘤-宿主相互作用和细胞动力学现象进行了成像，包括有丝分裂和凋亡性肿瘤细胞，与肿瘤细胞相互作用的基质细胞，肿瘤脉管系统，肿瘤血流以及药物反应。这种可成像的技术应导致体内癌细胞生物学的许多新见解。

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