• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes; 20080121-22; San Jose,CA(US) >Teal fluorescent proteins: Characterization of a reversibly photoswitchable variant
【24h】

Teal fluorescent proteins: Characterization of a reversibly photoswitchable variant

机译：蓝绿色荧光蛋白：可逆光开关变体的表征

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fluorescent proteins (FPs) emerged in the mid 1990s as a powerful tool for life science research. Cyan FPs (CFPs), widely used in multicolor imaging or as a fluorescence resonance energy transfer (FRET) donor to yellow FPs (YFPs), were considerably less optimal than other FPs because of some relatively poor photophysical properties. We recently initiated an effort to create improved or alternate versions of CFPs. To address the limitations of CFPs, an alternative known as monomeric teal FP1 (mTFP1) was engineered from the naturally tetrameric Clavularia CFP, by screening either rationally designed or random libraries of variants. mTFP1 has proven to be a particularly useful new member of the FP 'toolbox' by facilitating multicolor live cell imaging. During the directed evolution process of mTFP1, it was noticed that some earlier variants underwent fast and reversible photoisomerization. Some of the initial characterization of one particular mutant, designated as mTFP0.7, is described in this manuscript.
机译：荧光蛋白（FPs）于1990年代中期出现，成为生命科学研究的有力工具。青色FP（CFP）广泛用于多色成像或作为黄色FP（YFP）的荧光共振能量转移（FRET）供体，但由于其一些相对较差的光物理特性，其最佳性能却大大低于其他FP。我们最近开始努力创建CFP的改进版本或替代版本。为了解决CFP的局限性，通过筛选合理设计或随机的变体文库，从天然四聚体Clavularia CFP设计了一种称为单体蓝绿色FP1（mTFP1）的替代品。通过促进多色活细胞成像，mTFP1被证明是FP“工具箱”中特别有用的新成员。在mTFP1的定向进化过程中，注意到一些较早的变体经历了快速且可逆的光异构化。本手稿描述了一个特定的突变体，称为mTFP0.7的一些初始特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号