首页> 外文会议>Conference on Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing Ⅷ Jan 21-22, 2001, San Jose, USA >3-D imaging properties of a polarized light microscope revealed by birefringence measurements of small calcite crystals
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3-D imaging properties of a polarized light microscope revealed by birefringence measurements of small calcite crystals

机译:通过小方解石晶体的双折射测量揭示的偏光显微镜的3D成像特性

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We used a new type of polarized light microscope (Pol-Scope) equipped with a precision universal compensator to record images of small, uniaxial birefringent crystals. Pol-Scope measurements of small calcite crystals represent complete in-focus and out-of-focus retardance maps of the crystals. We relate the 3-D retardance maps to the crystal morphologies and the directions of their optic axes in three dimensional space. In addition to single crystal measurements, we also recorded 3-D retardance maps of small clusters of crystals to study the superposition of overlapping image features. We demonstrate that the retardance of overlapping image features in cluster images can be theoretically predicted by combining separately recorded single crystal images. Our experimental and theoretical analysis indicates that the superposition of retardance features is equivalent to adding incoherently the respective intensity values that were recorded with the Pol-Scope using different compensator settings. This analysis is part of a larger effort towards establishing 3-D image restoration procedures for polarized light imaging.
机译:我们使用一种配备有精密通用补偿器的新型偏振光显微镜(Pol-Scope)来记录小的单轴双折射晶体的图像。小方解石晶体的Pol-Scope测量代表了晶体的完整的焦点对准和焦点外的延迟图。我们将3-D延迟图与晶体形态及其在三维空间中的光轴方向相关联。除单晶测量外,我们还记录了小的晶体簇的3-D延迟图,以研究重叠图像特征的叠加。我们证明,通过组合单独记录的单晶图像,理论上可以预测簇图像中重叠图像特征的延迟。我们的实验和理论分析表明,延迟特性的叠加等同于使用不同的补偿器设置将Pol示波器记录的各个强度值不相干地相加。该分析是为偏振光成像建立3D图像恢复程序的一项较大工作的一部分。

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