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首页> 外文会议>Corrosion monitoring and measurement >Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor
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Performance of An Enzyme Electrode Designed for a Sulfide Monitoring Biosensor

机译：用于硫化物监测生物传感器的酶电极的性能

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摘要

The construction and evaluation of an enzyme electrode designed for a sulfide monitoring biosensor, are reported in this work. The enzyme, which is immobilized in a carbon (graphite) paste with 1,1 , dimeth-ylferrocene, catalyzes the oxidation of sulfide to sulfur.Amperometric measurements were carried out at room temperature, and a fixed potential of 0.3 V with reference to a saturated calomel electrode, using Tris-HCI buffer (pH 7.5) as the electrolyte, and sodium sulfide solution as the substrate. Under deaerated conditions, the biosensor responded linearly to the tested sulfide concentration range of 0-4mM, giving an average apparent Michaelis constant of 3mM, and maximum current responses between 0.7 and 3.6 μA. The sensitivity of the electrodes tested ranged from 0.2-0.3 μA mM~(-1).
机译：在这项工作中报道了设计用于硫化物监测生物传感器的酶电极的构建和评估。该酶固定在带有1,1，二甲基二茂铁的碳（石墨）糊状物中，催化硫化物氧化成硫，在室温下进行安培测量，固定电位为0.3 V，相对于饱和甘汞电极，使用Tris-HCl缓冲液（pH 7.5）作为电解质，硫化钠溶液作为底物。在脱气条件下，生物传感器对测试的硫化物浓度范围为0-4mM线性响应，平均表观米氏常数为3mM，最大电流响应为0.7至3.6μA。测试电极的灵敏度范围为0.2-0.3μAmM〜（-1）。

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  • 来源
    《Corrosion monitoring and measurement》|2007年|P.226-239|共14页
  • 会议地点 Norfolk VA(US)
  • 作者

    Reeta Sooknah; Sankara Papavinasam; Michael Attard; R. Winston Revie; W. Douglas Gould; Orlando Dinardo;

  • 作者单位

    CANMET Materials Technology Laboratory, Natural Resources Canada, 568 Booth Street, Ottawa, Ontario K1A 0G1, Canada;

    rnCANMET Materials Technology Laboratory, Natural Resources Canada, 568 Booth Street, Ottawa, Ontario K1A 0G1, Canada;

    rnCANMET Materials Technology Laboratory, Natural Resources Canada, 568 Booth Street, Ottawa, Ontario K1A 0G1, Canada;

    rnCANMET Materials Technology Laboratory, Natural Resources Canada, 568 Booth Street, Ottawa, Ontario K1A 0G1, Canada;

    rnCANMET Mining and Mineral Science Laboratories, Natural Resources Canada, 555 Booth Street, Ottawa, Ontario K1A 0G1, Canada;

    rnCANMET Mining and Mineral Science Laboratories, Natural Resources Canada, 555 Booth Street, Ottawa, Ontario K1A 0G1, Canada;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 材料腐蚀与保护;
  • 关键词

    sulfide monitoring; electrochemical biosensor; enzyme electrode; sulfide oxidase;

    机译：硫化物监测；电化学生物传感器酶电极硫化物氧化酶;

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