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首页> 外文会议>Defense and Security 2008: Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display >Rapid Screening and Species Identification of E. Coli, Listeria, and Salmonella by SERS Technique
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Rapid Screening and Species Identification of E. Coli, Listeria, and Salmonella by SERS Technique

机译：SERS技术快速筛选和鉴定大肠杆菌，李斯特菌和沙门氏菌

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Techniques for routine and rapid screening of the presence of foodborne bacteria are needed, and this study reports the feasibility of citrate-reduced silver colloidal SERS for identifying E. coli, Listeria, and Salmonella. Relative standard deviation (RSD) of SERS spectra from silver colloidal suspensions and ratios of P-O SERS peaks from small molecule (K_3PO_4) were used to assess the reproducibility, stability, and binding effectiveness of citrate-reduced silver colloids over batch and storage process. The results suggested the reproducibility of silver colloids over batch process and also stability and consistent binding effectiveness over 60-day storage period. Notably, although silver colloidal nanoparticles were stable for at least 90 days, their binding effectiveness began to decrease slightly after 60-day storage, with a binding reduction of about 12% at 90th day. Colloidal silver SERS, as demonstrated here, could be an important alternative technique in the rapid and simultaneous screening of the presence of three most outbreak bacteria due to the exclusive biomarkers, label-free and easy sampling attribute.
机译：需要常规和快速筛查食源性细菌存在的技术，这项研究报告了柠檬酸盐还原银胶体SERS鉴定大肠杆菌，李斯特菌和沙门氏菌的可行性。银胶体悬浮液的SERS光谱的相对标准偏差（RSD）和小分子（K_3PO_4）的P-O SERS峰的比率用于评估柠檬酸盐还原的银胶体在分批和储存过程中的重现性，稳定性和结合效果。结果表明银胶体在分批过程中具有可重现性，在60天的存储期内也具有稳定性和稳定的结合效果。值得注意的是，尽管银胶体纳米颗粒至少可以稳定90天，但在储存60天后其结合效果开始略有下降，在第90天时结合力下降了约12％。如此处所示，由于专有的生物标记，无标记且易于采样的特性，胶体银SERS可能是一种重要的替代技术，可用于同时快速筛查三种最具爆发性的细菌。

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