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A capillary-mimicking optical tissue phantom for diffuse correlation spectroscopy

机译:用于扩散相关光谱的模拟毛细管的光学组织体模

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Optical tissue phantoms are necessary for instrument benchmarking and providing a consistent baseline for experiments in various fields of tissue spectroscopy, including diffuse correlation spectroscopy (DCS). To provide the most useful comparisons, a phantom would ideally mimic tissue as closely as possible, including the geometry of static and dynamic scatterers. A branching design that keeps the capillary cross section constant ensures that the same flow velocity is found throughout the phantom while allowing for single input and output fittings to feed all of the capillaries simultaneously. The direction of each capillary is randomized every few millimeters by randomly allocating 2 by 2 "twisting" squares within each layer. These squares swap the locations of four adjacent artificial capillaries either clockwise or counterclockwise. Numerical simulations were used to verify the random walk-like behavior of the capillary paths resulting from this pattern. This is a step toward replicating the randomly varying directionality of actual capillaries. This design was verified by taking DCS measurements at different flow rates of Intralipid through the phantom, demonstrating the effect of the flow rate on the characteristic decay time of the autocorrelation.
机译:光学组织体模对于仪器基准测试是必不可少的,并为组织光谱学(包括扩散相关光谱法(DCS))的各个领域的实验提供一致的基线。为了提供最有用的比较,幻像在理想情况下会尽可能地模仿组织,包括静态和动态散射体的几何形状。保持毛细管横截面恒定的分支设计可确保在整个体模中发现相同的流速,同时允许单个输入和输出配件同时供入所有毛细管。通过在每层内随机分配2 x 2的“扭曲”正方形,每毛细管的方向每隔几毫米就随机化一次。这些正方形顺时针或逆时针交换四个相邻的人造毛细管的位置。数值模拟被用来验证由该模式引起的毛细管路径的随机步行样行为。这是朝着复制实际毛细管的随机变化方向的一步。通过在通过模体的脂质体内不同流速下进行DCS测量,验证了该设计,证明了流速对自相关特征衰减时间的影响。

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