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High-intensity UV LED Inactivation of Clostrium difficile Spores

机译：艰难梭菌孢子的高强度UV LED灭活

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Clostridium difficile spore transmission from contaminated surfaces is a continuing problem for health carefacilities. In this study high-intensity UV LED was used to rapidly inactivate Clostridium difficile spores.Wavelengths were specifically chosen to target protein stability through disulfide functional groups ratherthan nucleic acids.Clostridium difficile spores, at 3.09 x 10~6 CFU/ sq.in. carrier, were illuminated by hi-intensity UV LED.Irradiance is specified for the target distance, 25 mm. Spores were exposed to either 275nm alone or to a275nm+365nm combination for 30, 88, or 147 seconds. For 275nm alone, spores were exposed to 491.5mW/cm~2, 245.7 mW/cm~2, or 122.9 mW/cm~2. Irradiance varied for 365nm (720.3 mW/cm~2, 360.2 mW/cm~2, or180.1 mW/cm~2) while 275 irradiance was constant (245.7mW/cm~2) for the 275nm+365nm combination.High-intensity 275 nm UV LED was consistently effective at doses above 36.1 J/cm~2, the highest irradiance(491.5 mW/cm~2) achieved maximum log reduction at 14.7 J/cm~2. Addition of high-intensity 365 nm UV LEDresulted in maximum log reduction for all 365 nm intensities and doses tested. Maximum reduction of 5.79log was achieved.Simultaneous use of two specific UV wavelengths reduced time required for inactivation. This is consistentwith our previous findings on protein inactivation using RNase A~1, suggesting that targeting protein is viablefor inactivation of bacterial spores, even in the absence of wavelengths specifically targeting nucleic acids.
机译：从污染表面传播难辨梭状芽胞杆菌孢子一直是卫生保健的持续问题。在这项研究中，高强度紫外LED用于快速灭活艰难梭菌孢子。\ r \ n波长经过特别选择，可通过二硫键官能团而不是核酸来靶向蛋白质稳定性。\ r \ n艰难梭状芽胞杆菌孢子在3.09 x 10〜6 CFU /平方英寸\ r \ n辐照度被指定为目标距离25 mm。将孢子单独暴露于275nm或暴露于275nm + 365nm的组合中30、88或147秒。仅对于275nm，孢子暴露于491.5 \ r \ nmW / cm〜2、245.7 mW / cm〜2或122.9 mW / cm〜2。 365nm（720.3 mW / cm〜2、360.2 mW / cm〜2或\ r \ n180.1 mW / cm〜2）的辐照度变化，而275nm + 365nm的275辐照度是恒定的（245.7mW / cm〜2） \ r \ n在高于36.1 J / cm〜2的剂量下，高强度275 nm UV LED一直有效，最高辐照度（491.5 mW / cm〜2）在14.7 J / cm〜时实现了最大对数降低2。添加高强度365 nm紫外线LED可使所有365 nm强度和测试剂量的对数减少最大。达到最大减少量5.79 \ r \ nlog。\ r \ n同时使用两个特定的UV波长减少了灭活所需的时间。这与我们先前关于使用RNase A〜1进行蛋白质灭活的发现是一致的，这表明即使在不存在特异性针对核酸的波长的情况下，靶向蛋白质对于灭活细菌孢子也是可行的。

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