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首页> 外文会议>Diagnostic Optical Spectroscopy in Biomedicine IV; Progress in Biomedical Optics and Imaging; vol.8 no.41; Proceedings of SPIE-The International Society for Optical Engineering; vol.6628 >Multiphoton imaging and fluorescence lifetime studies on unstained cells and tissue at cryogenic conditions
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Multiphoton imaging and fluorescence lifetime studies on unstained cells and tissue at cryogenic conditions

机译：低温条件下未染色细胞和组织的多光子成像和荧光寿命研究

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摘要

Monitoring the functional status of cryo-preserved cells and tissue in-situ, I. E. in the frozen state, might allow for optimal adjustment of preservation conditions and might provide the information necessary to predict a functionality recovery rate. Here, an imaging approach with compositional sensitivity seems favourable. In our approach we use multiphoton microscopy in combination with fluorescence lifetime imaging to investigate cells, human and plant tissue at cryogenic conditions. By the non-linearity of multiphoton excitation we largely suppress image distortions attributed to scattering of incoming light. Only where the intensity of the pulsed near-infrared laser beam is sufficiently large, significant fluorescence is excited. This allows reaching penetration depth in ice comparable to the liquid state. As additional information we use the fluorescence decay to assign compositional entities. Results obtained on cells and tissues are discussed with respect to temperature dependencies and the related use for applications.
机译：监测冷冻保存的原位细胞和组织的功能状态，即处于冷冻状态的即大肠杆菌，可能允许对保存条件进行最佳调整，并可能提供预测功能恢复率所必需的信息。在这里，具有成分敏感性的成像方法似乎是有利的。在我们的方法中，我们将多光子显微镜与荧光寿命成像结合使用，以研究低温条件下的细胞，人和植物组织。通过多光子激发的非线性，我们在很大程度上抑制了归因于入射光散射的图像失真。仅在脉冲近红外激光束的强度足够大的情况下，才会激发显着的荧光。这允许达到与液态相当的冰中渗透深度。作为附加信息，我们使用荧光衰减来指定组成实体。关于温度依赖性及其在应用中的相关用途，讨论了在细胞和组织上获得的结果。

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