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首页> 外文会议>Optical diagnostics and sensing XVIII: Toward Point-of-Care Diagnostics >Phase Sensitive Diffraction Sensor for High Sensitivity Refractive Index Measurement
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Phase Sensitive Diffraction Sensor for High Sensitivity Refractive Index Measurement

机译：用于高灵敏度折射率测量的相敏衍射传感器

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In this study a diffraction based sensor has been developed for bio molecular sensing applications and performing assays in real time. A diffraction grating fabricated on a glass substrate produced diffraction patterns both in transmission and reflection when illuminated by a laser diode. We used zeroth order I(0.0) as reference and first order I(0.0) as signal channel and conducted ratiometric measurements that reduced noise by more than 50 times. The ratiometric approach resulted in a very simple instrumentation with very high sensitivity. In the past, we have shown refractive index measurements both for bulk and surface adsorption using the diffractive self-referencing approach. In the current work we extend the same concept to higher diffraction orders. We have considered order I(0.1) and Inland performed ratiometric measurements I(0.1)/I(1.1) to eliminate the common mode fluctuations. Since orders I(0,1) and I(1,1) behaved opposite to each other, the resulting ratio signal amplitude increased more than twice compared to our previous results. As a proof of concept we used different salt concentrations in DI water. Increased signal amplitude and improved fluid injection system resulted in more than 4 times improvement in detection limit, giving limit of detection 1.3×10~(-7) refractive index unit (RIU) compared to our previous results. The improved refractive index sensitivity will help significantly for high sensitivity label free bio sensing application in a very cost-effective and simple experimental set-up.
机译：在这项研究中，已开发出一种基于衍射的传感器，用于生物分子传感应用并实时执行测定。当用激光二极管照射时，在玻璃基板上制造的衍射光栅会在透射和反射中产生衍射图样。我们将零阶I（0.0）用作参考，并将一阶I（0.0）用作信号通道，并进行了比例测量，将噪声降低了50倍以上。比例法导致了非常简单的仪器，具有很高的灵敏度。过去，我们已经显示了使用衍射自参考方法测量体积和表面吸附的折射率。在当前的工作中，我们将相同的概念扩展到更高的衍射级。我们考虑了I（0.1）阶数和Inland执行的比率测量I（0.1）/ I（1.1），以消除共模波动。由于阶数I（0,1）和I（1,1）的行为彼此相反，因此所得到的比率信号幅度与我们之前的结果相比增加了两倍以上。作为概念验证，我们在去离子水中使用了不同的盐浓度。信号幅度的增加和流体注入系统的改进使检测极限提高了4倍以上，与我们以前的结果相比，检测极限为1.3×10〜（-7）折射率单位（RIU）。改进的折射率灵敏度将在非常经济高效且简单的实验设置中极大地帮助高灵敏度无标签生物传感应用。

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