Fabrication of spatially-patterned cells using selective adhesion on pre-structured fine particles

机译：使用对预制结构细颗粒的选择性粘附来制造空间图案化的单元

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The application of a neuron network to a bio/micro-sensor has potential as a drug screening device. In this study, micro-structures of surface-modified particles were applied to a scaffold for selective cell adhesion and growth. Silica particles were covered with a specific protein (fibronectin) or multi-walled carbon nanotubes (CNT) by electrostatic adsorption or transfer printing, respectively. They formed spatial patterns in a line-and-space structure tens of micrometers wide on a glass substrate. This paper investigates the effect of the coated material on the selectivity and adhesiveness of PC 12 phenochromocytoma cells. An incubation process causes PC 12 cells to autonomously align with selective adhesion on the micro-structures of both particles. The cells are minimally adhered to the glass surface around the particles. The structure of the fibronectin-coated particles enables a straight and uniform alignment of adhered cells, while that of bare silica particles causes randomly distributed cells. It was also found that the structure of CNT-adsorbed particles enhances cell adhesiveness to grow pseudopods of adhered cells.

机译：神经元网络在生物/微传感器上的应用具有作为药物筛选设备的潜力。在这项研究中，将表面改性颗粒的微结构应用于支架上，以进行选择性的细胞粘附和生长。二氧化硅颗粒分别通过静电吸附或转移印刷被特定的蛋白质（纤连蛋白）或多壁碳纳米管（CNT）覆盖。他们在玻璃基板上以几十微米宽的线和空间结构形成空间图案。本文研究了涂层材料对PC 12嗜铬细胞瘤细胞的选择性和粘附性的影响。孵育过程使PC 12细胞在两个颗粒的微结构上选择性粘附而自主排列。细胞最少地粘附在颗粒周围的玻璃表面上。纤连蛋白包被的颗粒的结构能够使粘附的细胞进行直线且均匀的排列，而裸露的二氧化硅颗粒的结构会导致细胞随机分布。还发现，CNT吸附颗粒的结构增强了细胞粘附性，以生长粘附细胞的假足。

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来源
《Emerging technology in precision engineering XIV》|2012年|615-620|共6页
会议地点 Hyogo(JP);Hyogo(JP)
作者
Arata Kaneko; Tatsuki Sugihara; Hiromichi Murakami; Iwori Takeda; Yasuhiro Tanaka; Nobuyuki Moronuki;
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作者单位

Tokyo Metropolitan University 6-6 Asahigaoka, Hino, Tokyo, Japan;

Tokyo Metropolitan University 6-6 Asahigaoka, Hino, Tokyo, Japan;

Tokyo Metropolitan University 6-6 Asahigaoka, Hino, Tokyo, Japan;

Tokyo Metropolitan University 6-6 Asahigaoka, Hino, Tokyo, Japan;

Tokyo Metropolitan University 6-6 Asahigaoka, Hino, Tokyo, Japan;

Tokyo Metropolitan University 6-6 Asahigaoka, Hino, Tokyo, Japan;

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原文格式 PDF
正文语种 eng
中图分类
关键词
adhesion; cell; particles; self-assembly; CNT; protein; scaffold;

机译：附着力细胞;粒子;自组装；碳纳米管;蛋白;脚手架;

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1. Fabrication of Stacked Layers of Fine Particle Assembly Aiming at Wavelength-Selective Reflection [J] . Kenta Wakamatsu, Nobuyuki Moronuki International journal of automation technology . 2016,第6期

机译：针对波长选择性反射的细颗粒组件堆叠层的制备
2. Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion [J] . Iwori TAKEDA, Shogo SERIZAWA, Arata KANEKO Mechanical Engineering Journal . 2016,第1期

机译：使用自组装颗粒的微结构支架的制备以及表面几何形状对细胞粘附的影响
3. VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro [J] . Hong Yang, Fenglong Zhao, Ying Li, International Journal of Nanomedicine . 2013,第1期

机译：以VCAM-1为靶点的核/壳纳米粒子，可在剪切流和细胞核磁共振成像下选择性粘附并递送至脂多糖诱导的炎症的内皮细胞
4. Fabrication of spatially-patterned cells using selective adhesion on pre-structured fine particles [C] . Arata Kaneko, Tatsuki Sugihara, Hiromichi Murakami International Conference on Precision Engineering . 2012

机译：使用在预结的细颗粒上使用选择性粘合来制造空间图案细胞
5. Pressure gradient and particle adhesion in the pneumatic transport of fine particles. [D] . Wang, Fujing. 1997

机译：气动颗粒输送中的压力梯度和颗粒附着力。
6. VCAM-1-targeted core/shell nanoparticles for selective adhesion and delivery to endothelial cells with lipopolysaccharide-induced inflammation under shear flow and cellular magnetic resonance imaging in vitro [O] . Hong Yang, Fenglong Zhao, Ying Li, 2013

机译：以VCAM-1为靶点的核/壳纳米粒子在体外剪切流和细胞核磁共振成像下选择性粘附并递送至脂多糖诱导的炎症的内皮细胞
7. FINE STRUCTURAL ALTERATIONS IN CELL PARTICLES DURING CHEMICAL CARCINOGENESIS : III. Selective Action of Hepatic Carcinogens Other than 3'-Methyl-4-Dimethylaminoazobenzene on Different Types of Mitochondrial Swelling. Effect of Stimulated Liver Growth [O] . Arcos, Joseph C., Gosch, Hans H., Zickafoose, David 1961

机译：化学致癌过程中细胞颗粒的精细结构改变：III。 3'-甲基-4-二甲基氨基偶氮苯以外的肝致癌物对不同类型线粒体肿胀的选择性作用。刺激肝脏生长的影响

Fabrication of spatially-patterned cells using selective adhesion on pre-structured fine particles

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