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首页> 外文会议>Endoscopic Microscopy II; Progress in Biomedical Optics and Imaging; vol.8 no.9; Proceedings of SPIE-The International Society for Optical Engineering; vol.6432 >Design of a multi-spectral channel for in-vivo confocal microscopy
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Design of a multi-spectral channel for in-vivo confocal microscopy

机译：体内共聚焦显微镜的多光谱通道设计

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摘要

We present a modified multi-spectral configuration of a slit-scanning confocal microendoscope that provides higher spectral resolution in a fully automated interface. Tissue fluorescence signal is directed through a dispersive element that spreads the spectral information across the CCD camera mapping spectral information perpendicular to the confocal slit. The dispersive element may be chosen to meet the specific requirements defined by the user. Our current system uses a BK7 prism with a 45° wedge angle and a 20mm diameter clear aperture. The prism is shifted from the optical axis allowing automated switching from grayscale (beam on-axis) to multi-spectral (beam off-axis) imaging by tilting a computer controlled mirror. The system records over a spectral range of 450nm to 750nm. The minimum resolvable wavelength difference varies from 2.1nm to 8.3nm over the spectral range. The lateral and axial resolution of the system is approximately 3 μm by 30 μm, respectively, and is the same for both grayscale and multi-spectral imaging modes. Multi-spectral imaging results from ex-vivo tissues are presented.
机译：我们提出了狭缝扫描共聚焦显微内窥镜的改进的多光谱配置，该配置在全自动界面中提供了更高的光谱分辨率。组织荧光信号被引导通过一个色散元件，该色散元件将光谱信息散布在CCD相机上，从而映射垂直于共焦缝隙的光谱信息。可以选择分散元件以满足用户定义的特定要求。我们当前的系统使用具有45°楔角和20mm直径透明孔径的BK7棱镜。棱镜从光轴移开，通过倾斜计算机控制的反射镜，可以自动从灰度（光束在轴上）切换到多光谱（光束在轴外）成像。该系统记录的光谱范围为450nm至750nm。在光谱范围内，最小可分辨波长差在2.1nm至8.3nm之间变化。系统的横向和轴向分辨率分别约为3μmx 30μm，并且对于灰度和多光谱成像模式都是相同的。呈现了来自离体组织的多光谱成像结果。

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