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Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using asingle-mode fibre

机译：迈向非线性内窥镜检查探索肺气道：使用单模光纤对人类肺组织进行初步光谱学研究

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We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.

机译：我们正在开发一种非线性纤维内窥镜，用于在支气管镜检查过程中对细胞外基质胶原蛋白和弹性蛋白原纤维网络进行成像。作为概念的证明，已使用特殊设计的砂线用于色散补偿，获得了标准的2米长单模光纤输出处的激光脉冲，脉冲持续时间约为50 fs，脉冲能量高达50 uJ。利用这些脉冲，我们对非线性内源性信号进行了光谱表征，该信号由双光子荧光和二次谐波生成组成，并来源于不同厚度的人肺组织，在信号采集的前向和后向几何结构中均受到激发在830 nm

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《Endoscopic microscopy VI》|2011年|p.789309.1-789309.6|共6页
会议地点 San Francisco CA(US)
作者
Donald A. Peyrot; Claire Lefort; Darine Abi Haidar; Tigran Mansuryan; Sergei Kruglik; et al;
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作者单位

ANBioPhy - FRE 3207, Universite Pierre et Marie Curie,4 place Jussieu - tour 42-43, 75252 Paris cedex, France.;

XLIM - UMR CNRS 6172, Universite de Limoges,123 avenue Albert Thomas, 87060 Limoges cedex, France.;

ANBioPhy - FRE 3207, Universite Pierre et Marie Curie,4 place Jussieu - tour 42-43, 75252 Paris cedex, France.;

XLIM - UMR CNRS 6172, Universite de Limoges,123 avenue Albert Thomas, 87060 Limoges cedex, France.;

ANBioPhy - FRE 3207, Universite Pierre et Marie Curie,4 place Jussieu - tour 42-43, 75252 Paris cedex, France.;

et al;

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原文格式 PDF
正文语种 eng
中图分类医用物理学;
关键词
Endomicroscopy; bronchoscopy; spectroscopy; lung tissue; dispersion compensation; twophoton fluorescence; second harmonic generation;

机译：内窥镜检查；支气管镜;光谱学肺组织;色散补偿；双光子荧光二次谐波产生;

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Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using asingle-mode fibre

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