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首页> 外文会议>European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE >Enrichment of MTBE degrading bacteria from gasoline contaminated subsurface soil
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Enrichment of MTBE degrading bacteria from gasoline contaminated subsurface soil

机译：汽油污染的地下土壤中MTBE降解细菌的富集

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Biodegradation of methyl tert-butyl ether (MTBE) in aquifer material was studied during slurry enrichments (batch-systems) simulating in situ conditions. Soil samples taken at 4 different depths at a gasoline-contaminated site were used and the microbial population of each soil sample was separately enriched. The MTBE-degradation kinetics and the community dynamics as a response to different implemented in situ important parameters (e.g. additions of nutrients, addition of extra oxygen and presence of co-contaminants) were determined and compared between the different depths. In all enrichments the MTBE-biodegradation capacity was active for more than 600 days. PCR-DGGE fingerprints revealed that addition of extra nutrients or benzene did not lead to significant changes in the eubacterial population. No differences could also be observed between the community composition of the enrichment conditions after 1 year of incubation at different oxygen concentrations (8, 9.5 and 11.5 mg/l DO). In contrast, adding propane did induce differences in band intensity of the fingerprint. A different eubacterial community was observed at the 4 different examined depths.
机译：在模拟现场条件的淤浆浓缩（间歇系统）过程中，研究了含水层材料中甲基叔丁基醚（MTBE）的生物降解。使用在汽油污染的地点在4个不同深度处采集的土壤样品，并分别富集每个土壤样品的微生物种群。确定并比较了不同深度之间MTBE降解动力学和群落动力学对不同实地实施的重要参数（例如添加营养，添加额外的氧气和存在的污染物）的影响。在所有浓缩中，MTBE的生物降解能力均超过600天。 PCR-DGGE指纹图谱表明，添加额外的营养素或苯不会导致细菌总数的显着变化。在不同氧气浓度（8、9.5和11.5 mg / l DO）下孵育1年后，富集条件的群落组成之间也没有观察到差异。相反，添加丙烷的确会引起指纹谱带​​强度的差异。在4个不同的检查深度处观察到了不同的真细菌群落。

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