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首页> 外文会议>European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE >Effect of MTBE on growth and metabolism of degrading microorganisms
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Effect of MTBE on growth and metabolism of degrading microorganisms

机译：MTBE对降解微生物生长代谢的影响

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摘要

Methyl tert-butyl ether (MTBE) is a synthetic compound that was developed as a technological solution to a technology-derived problem created by air pollution from vehicle emissions. Because of its undesirable effects on drinking water and ecologically harmful effects, MTBE removal has become a public health and environmental concern. Potential method used for MTBE removal from soils and aquifers is microbial decomposition. Genotoxicity of MTBE was monitored by Ames test with Salmonella typhimurium his~- (TA98, TA 100, YG1041, YG1042) as indicator strains. Concentrations of MTBE/plate tested were 3.0 mg and1.5mg. Obtained results did not prove the MTBE genotoxicity. Among the MTBE aerobic degradation products of indigenous bacterial strains tert-butyl alcohol (TBA) was found using GC. The genotoxicity of TBA was tested using the identical system. All so far measured data indicate that MTBE will be cometabolically oxidized by monooxygenase (MO) enzyme activity that is induced by oxidation of n-alkanes under aerobic conditions. The MO enzyme requires molecular oxygen to oxidize the target chemical; this process is called β-oxidation and is common mechanism for n-alkane biodegradation. Its role in MTBE biodegradation is evaluated.
机译：甲基叔丁基醚（MTBE）是一种合成化合物，是为解决因汽车排放造成的空气污染而导致的技术衍生问题的技术解决方案而开发的。由于其对饮用水的不良影响和对生态的不利影响，MTBE的去除已成为公众健康和环境问题。从土壤和含水层中去除MTBE的潜在方法是微生物分解。以鼠伤寒沙门氏菌his-（TA98，TA 100，YG1041，YG1042）为指示菌株，通过Ames试验监测MTBE的基因毒性。所测试的MTBE /板的浓度为3.0mg和1.5mg。获得的结果并未证明MTBE的遗传毒性。使用GC发现了本地细菌菌株的叔丁醇（TBA）有氧降解产物。使用相同的系统测试了TBA的遗传毒性。到目前为止所有测量数据表明，MTBE将被有氧条件下的正构烷烃氧化诱导的单加氧酶（MO）酶代谢而被氧化。 MO酶需要分子氧来氧化目标化学物质。此过程称为β-氧化，是正构烷烃生物降解的常见机制。评估其在MTBE生物降解中的作用。

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