• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE >Monitoring of dehalogenating communities in contaminated soils and in combined anaerobic/aerobic soil biotreatment reactors
【24h】

Monitoring of dehalogenating communities in contaminated soils and in combined anaerobic/aerobic soil biotreatment reactors

机译：监测污染土壤和厌氧/好氧土壤生物处理反应器中的脱卤群落

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We are evaluating the use of sequence markers specific for dehalogenating species to assess the dehalogenating potential of contaminated soils and to optimize soil bioremediation process parameters. Two systems contaminated with toxic and persistent chlorinated hydrocarbons have been investigated: a soil contaminated by dichloroethane (DCA) and a laboratory-scale, sequential anaerobic-aerobic process for the treatment of a soil contaminated with DDTs, chlorinated benzenes (CBs) and heavy metals, (mainly Hg and As). The microbial population profile of samples from these soils was analyzed by ARISA (Amplified Ribosomal Intergenic Spacer Analysis) and DGGE (Denaturing gradient gel electrophoresis) of 16S rKNA genes. DGGE band sequencing detected the presence of bacteria previously associated with soils polluted by halogenated hydrocarbons or present in consortia active in the removal of these compounds. A PCR specific assay using primers for Dehalococcoides sp. showed the presence of this genus in the anaerobic phase of the soil treatment process.
机译：我们正在评估使用特定于脱卤物种的序列标记来评估受污染土壤的脱卤潜力并优化土壤生物修复工艺参数。已经研究了两种被有毒和持久性氯代烃污染的系统：一种被二氯乙烷（DCA）污染的土壤，以及一种实验室规模的顺序厌氧-好氧工艺，用于处理被滴滴涕，氯化苯（CBs）和重金属污染的土壤。 ，（主要是汞和砷）。通过16S rKNA基因的ARISA（核糖体间基因间隔扩增）和DGGE（变性梯度凝胶电泳）分析了这些土壤样品的微生物种群概况。 DGGE谱带测序检测到先前与被卤代烃污染的土壤有关的细菌的存在，或存在于对这些化合物具有去除作用的联合体中的细菌。使用Dehalococcoides的引物进行PCR特异性检测。表明该属存在于土壤处理过程的厌氧阶段。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号