Monitoring sulfate-reducing bacteria (SRB) using molecular tools during in situ immobilization of heavy metals

机译：在原位固定重金属过程中使用分子工具监测硫酸盐还原细菌（SRB）

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In situ bioprecipitation (ISBP) of heavy metals as metal sulfides by the activity of sulfate-reducing bacteria (SRB) is a promising strategy for sustaining groundwater quality. The feasibility of the ISBP process for a Zn, Cd, Ni and Co-polluted industrial site was studied in column experiments, using lactate, molasses or HRC~(~R) as supplemented electron donor and C-source. In addition, the effect of extra N/P was evaluated. In order to link the results of analytical analyzes to SRB-community composition and activity, the SRB population was monitored by molecular tools such as SRB-subgroup or - genus specific PCR and DGGE of the 16S rRNA- and dsr (dissimilatory sulfite reductase) gene.

机译：通过减少硫酸盐的细菌（SRB）的活性，将重金属作为金属硫化物进行原位生物沉淀（ISBP）是维持地下水质量的一种有前途的策略。利用乳酸，糖蜜或HRC〜（〜R）作为补充电子供体和碳源，在柱实验中研究了Zn，Cd，Ni和Co污染工业现场ISBP工艺的可行性。另外，评估了额外N / P的作用。为了将分析结果与SRB社区组成和活性联系起来，通过分子工具（例如SRB亚组或16S rRNA-和dsr（异亚硫酸盐还原酶）基因的特异性PCR和DGGE）监测了SRB种群。

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来源
《European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE》|2004年|P.569-572|共4页
会议地点 Oostende(BE);Oostende(BE)
作者
J. Geets; B. Borremans; K. Vanbroeckhoven; L. Diels; D. Van Der Lelie; J. Vangronsveld;
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Vito (Vlaamse Instelling voor Technologisch Onderzoek), Mol, Belgium;

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原文格式 PDF
正文语种 eng
中图分类环境生物学;
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1. PCR-DGGE and Real-time PCR dsrB-based Study of the Impact of Heavy Metals on the Diversity and Abundance of Sulfate-reducing Bacteria [J] . Islamud-Din, Abd El-Latif Hesham, Ayaz Ahmad, Biotechnology and bioprocess engineering . 2014,第4期

机译：基于PCR-DGGE和实时PCR dsrB的重金属对硫酸盐还原菌多样性和丰度影响的研究
2. Corrosion characteristics of sulfate-reducing bacteria (SRB) and the role of molecular biology in SRB studies: an overview [J] . Anandkumar Balakrishnan, George Rani P., Maruthamuthu Sundaram, Corrosion Reviews . 2016,第1a2期

机译：硫酸盐还原细菌（SRB）的腐蚀特征以及分子生物学在SRB研究中的作用：概述
3. Depth-related coupling relation between methane-oxidizing bacteria (MOBs) and sulfate-reducing bacteria (SRBs) in a marine sediment core from the Dongsha region, the South China Sea [J] . Xu Xiao-Ming, Fu Shao-Ying, Zhu Qing, Applied Microbiology and Biotechnology . 2014,第24期

机译：南海东沙海域沉积物中甲烷氧化细菌与硫酸盐还原细菌深度相关的耦合关系
4. Monitoring sulfate-reducing bacteria (SRB) using molecular tools during in situ immobilization of heavy metals [C] . J. Geets, B. Borremans, K. Vanbroeckhoven, European symposium on environmental biotechnology . 2004

机译：使用分子工具在原位固定的重金属中监测减少硫酸盐的细菌（SRB）
5. Effects of iron on the generation of hydrogen sulfide in a mixed culture containing sulfate-reducing bacteria (SRB) and methane-producing bacteria (MPB) [D] . Pollett, Haemi 2003

机译：铁对含有硫酸盐还原细菌（SRB）和产甲烷细菌（MPB）的混合培养物中硫化氢生成的影响
6. Quantifying Heavy Metals Sequestration by Sulfate-Reducing Bacteria in an Acid Mine Drainage-Contaminated Natural Wetland [O] . John W. Moreau, John H. Fournelle, Jillian F. Banfield 2013

机译：在酸性矿山排水污染的自然湿地中通过硫酸盐还原细菌对重金属的固存进行定量
7. Molecular genetic analysis of the composition of the microbial community in the flood water of the Königstein mine and detection of sulfate-reducing bacteria (SRB) [O] . Rommel Liana 2011

机译：Königstein矿山洪水中微生物群落的分子遗传分析及硫酸盐还原菌（sRB）的检测
8. ESEM/EDS, SEM/EDS and EIS Studies of Coated 4140 Steel Exposed to Marine, MixedMicrobial Communities Including Sulfate-Reducing Bacteria (SRB). (Reannouncement with New Availability Information) [R] . Jones-Meehan, J., Walch, M., Little, B. J., 1992

机译：EsEm / EDs，sEm / EDs和EIs研究涂层4140钢暴露于海洋，混合微生物群落，包括硫酸盐还原菌（sRB）。（重新公布新的可用性信息）

Monitoring sulfate-reducing bacteria (SRB) using molecular tools during in situ immobilization of heavy metals

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