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Sensitive fluorescence detection with microstructured optical fibers

机译：微结构光纤的灵敏荧光检测

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摘要

Optical fibers are ideal for environmental sensing applications because of their ability to transmit optical signals to and from the sensing region without the use of free-space optics. By accessing the evanescent field, the fiber itself can act as the sensing element and long interaction lengths can be achieved. Microstructured optical fibers are particularly suited to such applications as the species of interest can occupy the air spaces within the fiber. Here we use a suspended nanowire design that provides the high evanescent overlap of a nanowire with the long interaction length and robustness of a conventional fiber. The fluorescence-detection approach proposed in this paper is attractive because of its simplicity. When one end of the fiber is dipped into the sample, capillary forces draw the liquid into the voids within the fiber. The evanescent field of the pump light excites the fluorescent labels and a portion of the fluorescence is captured by the fiber core and propagates to the fiber tips. The aim of this work is to improve the sensing architecture to increase the sensitivity of the sensor, and to examine the primary factors such as the background glass fluorescence currently restricting the detection limit in this type of sensor. This work is focused on biological detection in liquid samples using Quantum dots, but through careful selection of the fluorescent dye this can be extended to a large number of alternative applications. Using this system we are able to detect quantum dot concentrations as low as 10 pM.
机译：光纤是环境感应应用的理想选择，因为它们能够在不使用自由空间光学器件的情况下，将光信号往返于感应区域进行传输。通过进入the逝场，光纤本身可以充当传感元件，并且可以实现长的相互作用长度。微结构光纤特别适合于此类应用，因为感兴趣的物种可能会占据光纤内的空气空间。在这里，我们使用了悬浮的纳米线设计，该设计提供了纳米线的高渐逝性重叠以及传统纤维的长相互作用长度和坚固性。由于其简单性，本文提出的荧光检测方法很有吸引力。当纤维的一端浸入样品中时，毛细作用力将液体吸入纤维内的空隙中。泵浦光的渐逝场激发荧光标记，并且一部分荧光被纤维芯捕获并传播到纤维尖端。这项工作的目的是改善感测体系结构，以提高传感器的灵敏度，并检查主要因素，例如当前限制此类传感器检测极限的背景玻璃荧光。这项工作的重点是使用量子点对液体样品进行生物检测，但是通过仔细选择荧光染料，可以将其扩展到大量其他应用中。使用该系统，我们能够检测到低至10 pM的量子点浓度。

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