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Understanding the role of hemoglobin in altering multilayered tissue intrinsic fluorescence in the visible region using fiber optics

机译:使用光纤了解血红蛋白在改变可见区域中多层组织固有荧光中的作用

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Extraction of intrinsic fluorescence in a turbid medium such as tissue is a challenging problem due to the various interrelationships between different constituents of tissue. If the fluorescence happens to be in the visible region, predominant absorption of hemoglobin can quench the emission making the intrinsic extraction and hence the actual measurement of the respective fluorophore concentration erroneous. This work explores to understand the effect of hemoglobin quenching in layered skin tissue phantoms. Monte Carlo simulations on layered phantoms were performed with varying concentrations of FAD (Flavin Adenine Dinucleotide) and hemoglobin and the results are correlated with experimental fluorescence measurements.
机译:由于组织不同成分之间的各种相互关系,在诸如组织之类的混浊介质中提取固有荧光是一个具有挑战性的问题。如果荧光恰好在可见光区域,则血红蛋白的主要吸收会猝灭发射,从而导致固有提取,因此相应荧光团浓度的实际测量是错误的。这项工作旨在了解了解血红蛋白猝灭在分层皮肤组织模型中的作用。使用不同浓度的FAD(黄素腺嘌呤二核苷酸)和血红蛋白对分层体模进行了Monte Carlo模拟,其结果与荧光实验值相关。

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