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首页> 外文会议>Fifth International Conference on Ion Exchange Processes held on 5-9 July 1998 at Plas Coch College, The North East Wales Institute, Wrexham, UK >Polygran sorbents for separation of protein mixtures
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Polygran sorbents for separation of protein mixtures

机译：用于分离蛋白质混合物的Polygran吸附剂

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摘要

Among methods used in the separation and fractionation of biologically active compounds, in particular proteins, one of the leading positions is still occupied by ion exchange chromatorgraphy.~1 Experimental experience acquired during many years of application of the method in biotechnology allows us to predict optimum conditions of the separation of components of complex biological mixtures and to choose ion exchange supports possessing properties required for the purpose. However, in spite of the success achieved, there is a number of problems due to the slow kinetics of sorption and incomplete desorption of compounds sorbed from polymer supports. Solutions to these problems, and the overal effectiveness of the chromatography, depend predominantly on properties of the support, and this is why the search for new sorbents with improved chromatographic characteristics has to be continued.~2
机译：在用于分离和分离生物活性化合物（尤其是蛋白质）的方法中，离子交换色谱法仍占据着领先地位。〜1在生物技术中多年应用该方法获得的实验经验使我们能够预测最佳复杂生物混合物成分分离的条件以及选择具有目的所需性能的离子交换载体。然而，尽管取得了成功，但是由于从聚合物载体上吸附的化合物的缓慢的吸附动力学和不完全的解吸，仍然存在许多问题。这些问题的解决方案以及色谱的总体有效性主要取决于载体的性质，这就是为什么必须继续寻求具有改善色谱特性的新吸附剂的原因。〜2

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