Microscopy using randomized speckle illumination

机译：显微镜使用随机散斑照明

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It is well known for structured illumination microscopy (SIM) that the lateral resolution by a factor of two beyond the classical diffraction limit is achieved using spatially structured illumination in wide-field fluorescence microscope, In the state of art SIM systems, grating patterns are generally generated by physical gratings or by spatial light modulators such as digital micro mirrors (DMD), liquid crystal displays (LCD). In this study, using a combination of LCD and ground glasses, size controlled randomized speckle patterns are generated as an illumination source for the microscope. Proof of concept of using speckle illumination in SIM configuration is tested by imaging fixed BPAE cells.

机译：对于结构照明显微镜（SIM）而言，众所周知的是，在宽场荧光显微镜中使用空间结构照明可实现超过经典衍射极限两倍的横向分辨率。在现有技术的SIM系统中，光栅图案通常是由物理光栅或空间光调制器（如数字微镜（DMD），液晶显示器（LCD））产生。在这项研究中，结合使用LCD和毛玻璃，可以生成尺寸受控的随机散斑图案，作为显微镜的照明源。通过对固定的BPAE电池成像，测试了在SIM配置中使用斑点照明的概念验证。

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来源
《Fifth International Conference on Optical and Photonics Engineering》|2017年|104490H.1-104490H.5|共5页
会议地点 Singapore(SG)
作者
Sandeep M. Perinchery; Anant Shinde; V.M. Murukeshan;
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作者单位

Center for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798;

Center for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798;

Center for Optical and Laser Engineering, School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798;

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原文格式 PDF
正文语种 eng
中图分类
关键词
Speckle; Structure illumination; Fluorescence microscopy; Optical imaging;

机译：斑点;结构照明；荧光显微镜光学成像;
入库时间 2022-08-26 14:02:08

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1. Optical sectioning enhancement using higher-order moment signals in random speckle-structured illumination microscopy [J] . Vigoren Andrew, Zavislan James M. Journal of the Optical Society of America, A. Optics, image science, and vision . 2018,第3期

机译：在随机散斑结构照明显微镜中使用高阶时刻信号进行光学切片增强
2. Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations [J] . Negash Awoke, Labouesse Simon, Sandeau Nicolas, Journal of the Optical Society of America, A. Optics, image science, and vision . 2016,第6期

机译：使用随机散斑照明提高三维荧光显微镜的轴向和横向分辨率
3. Two-wavelength digital holographic microscopy using speckle illuminations [J] . Funamizu Hideki, Sugata Kota, Uozumi Jun, Optics and Lasers in Engineering . 2020,第May期

机译：双波长数字全息显微镜使用斑点照明
4. Microscopy using randomized speckle illumination [C] . Sandeep M. Perinchery, Anant Shinde, V.M. Murukeshan International Conference on Optical and Photonics Engineering . 2017

机译：使用随机斑点照明的显微镜故障
5. Optically Sectioned Imaging of Microvasculature of In-Vivo and Ex-Vivo Thick Tissue Models with Speckle-illumination HiLo Microscopy and HiLo Image Processing Implementation in MATLAB Architecture. [D] . Suen, Ricky Wai. 2013

机译：使用斑点照明HiLo显微镜和HiLo图像处理在MATLAB体系结构中实现的体内和体外的厚组织模型的微脉管的光学切片成像。
6. Speckle-structured illumination for 3D phase and fluorescence computational microscopy [O] . Li-Hao Yeh, Shwetadwip Chowdhury, Nicole A. Repina, 2019

机译：用于3D相的斑点结构照明和荧光计算显微镜
7. Speckle-structured illumination for 3D phase and fluorescence computational microscopy [O] . Li-Hao Yeh, Shwetadwip Chowdhury, Nicole A. Repina, 2019

机译：3D相和荧光计算显微镜的斑点结构照明

Microscopy using randomized speckle illumination

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