THE EFFECT OF SERUM CONCENTRATION ON THE DNA SYNTHESIS OF SMMC-7721 CELLS

机译：血清浓度对SMMC-7721细胞DNA合成的影响

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Serum free or short-time serum free cell culture was one of the most important method for studying cell growth, control and metabolism, especially for the study of growth factors and drugs [1,2,3]. In the study of HGF, we found that short time serum-free culture could stimulate greatly the incorporation of ~8H-TdR of SMMC-7721 cells [4]. This was important for judging the effect of growth factors correctly. But the result was different from that of Zhang [5]in the study of lymphocytes. In order to clarify the effect of short-time serum free on the DNA synthesis, the incorporation of ~8H -TdR of SMMC-7721 cells cultured in RPMI 1640 media containing different concentration of serum or non serum was studied.

机译：无血清或短期无血清细胞培养是研究细胞生长，控制和代谢的最重要方法之一，特别是对于生长因子和药物的研究[1,2,3]。在HGF的研究中，我们发现短时间无血清培养可以极大地刺激SMMC-7721细胞的〜8H-TdR掺入[4]。这对于正确判断生长因子的作用很重要。但是在淋巴细胞研究中，该结果与Zhang [5]的结果不同。为了阐明短时间游离血清对DNA合成的影响，研究了在含有不同浓度血清或非血清的RPMI 1640培养基中培养的SMMC-7721细胞〜8H -TdR的掺入。

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来源
《First international symposium on bioanalytical chemistry》|1995年|183-184|共2页
会议地点 Xian(CN);Xian(CN)
作者
Jiang Shiming; Xu ZHaohui; Zhang Zhenyu;
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Biology Department, Shandong Normal University, Jinan Shandong (250014), PR China;

Biology Department, Shandong Normal University, Jinan Shandong (250014), PR China;

Biology Department, Shandong Normal University, Jinan Shandong (250014), PR China;

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机译：血清浓度对SMMC-7721细胞DNA合成的影响
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7. Serum deprivation enhances DNA synthesis of human hepatoma SMMC-7721 cells* [O] . Shi-Ming Jiang 1998

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THE EFFECT OF SERUM CONCENTRATION ON THE DNA SYNTHESIS OF SMMC-7721 CELLS

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