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首页> 外文会议>First international symposium on bioanalytical chemistry >PCRTOOL: A Software Package to Analyse the Data and Design Primers for PCR
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PCRTOOL: A Software Package to Analyse the Data and Design Primers for PCR

机译：PCRTOOL：用于分析PCR数据和设计引物的软件包

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摘要

A computer software package called PCRTOOL is written in Turbo C/ M ASM and DOS based. It is suitable for IBM compatible computer(640k RAM , DOS 3. 30 or later; harddisk ;VGA graphics adapter). Providing a dialog-based natural language interface. Two language version can be obtained (Chinese or English). The PCR TOOL can be used to design oligonucleotide primers for neucleotid acid sequences in Genebank files or ASCII files , and evaluated any user-defined sequence as a primer upon length , span and Tm value. Other PCR related Analysis can also be accomplished by PCRTOOL, including a) the homology analysis between two nucleotide acid sequences , b)the restriction sites location in any given sequence, c)the translation of given nucleotid sequences to amino acid sequences, instantaneously figure out the percentage of every kind of amino acid and essentialon-essential anino acid. All the analysis results can be displayed on screen, saved as a file or printed.
机译：用Turbo C / MASM和DOS编写了一个称为PCRTOOL的计算机软件包。它适用于IBM兼容计算机（640k RAM，DOS 3. 30或更高版本；硬盘； VGA图形适配器）。提供基于对话框的自然语言界面。可获得两种语言版本（中文或英文）。 PCR TOOL可用于为Genebank文件或ASCII文件中的核苷酸序列设计寡核苷酸引物，并根据长度，跨度和Tm值评估任何用户定义的序列作为引物。其他与PCR相关的分析也可以通过PCRTOOL完成，包括a）两个核苷酸序列之间的同源性分析，b）任何给定序列中的限制位点位置，c）给定核苷酸序列向氨基酸序列的翻译，立即找出每种氨基酸和必需/非必需氨基酸的百分比。所有分析结果都可以显示在屏幕上，另存为文件或打印。

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  • 来源
  • 会议地点 Xian(CN);Xian(CN)
  • 作者

    Ouyang Hongsheng; Ruan Chcngmai; Li Chengyao; Guan Jiang; Zhang Yujing; Wang Yusong;

  • 作者单位

    Department of Biochemistry, Changchun University of Agriculture and Animal Science, 175 Xian Road, Changchun 130062, P.R.China;

    Department of Biochemistry, Changchun University of Agriculture and Animal Science, 175 Xian Road, Changchun 130062, P.R.China;

    Department of Biochemistry, Changchun University of Agriculture and Animal Science, 175 Xian Road, Changchun 130062, P.R.China;

    Department of Biochemistry, Changchun University of Agriculture and Animal Science, 175 Xian Road, Changchun 130062, P.R.China;

    Department of Biochemistry, Changchun University of Agriculture and Animal Science, 175 Xian Road, Changchun 130062, P.R.China;

    Department of Biochemistry, Changchun University of Agriculture and Animal Science, 175 Xian Road, Changchun 130062, P.R.China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 一般性问题;
  • 关键词

    PCR; computer programing;

    机译：PCR;电脑程式设计;

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