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首页> 外文会议>Laser Florence 2017: Advances in Laser Medicine >Differential expression of myofibroblasts on CO2 Laser wounds and scalpel wounds: an experimental model
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Differential expression of myofibroblasts on CO2 Laser wounds and scalpel wounds: an experimental model

机译：肌成纤维细胞在CO2激光伤口和手术刀伤口上的差异表达：实验模型

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Wound contraction of both traumatic and surgical origin may reduce or limit the function of the tissue. Myofibroblasts are cells involved on the process of wound contraction, which is smaller on CO_2 Laser wounds. The aims of this study were to quantitative and statistically assess the presence of myofibroblasts on both conventional and CO_2 Laser wounds. Thirty-two animals (rattus norvegicus) were divided into four groups and operated using either the CO_2 Laser (groups A1 and A2) or conventional scalpel (groups B1 and B2). The animals were sacrificed eight days post-operatively (groups: A1 and B1) and 14th days after surgery (groups: A2 and B2). The spec imens we re routinely processed to wax and stained with a-Smooth Muscle Actin (aSMA) and analyzed under light microscopy (40X). Two standard areas around the wound of each slide were selected and used to count the number of myofribroblasts present using a calibrated eyepiece and a graticule. The number of myofibroblasts at day eight was significantly higher than at day 14Th. Comparison of the two techniques at day eight showed significant differences between the two groups (Laser, p=0.007 and scalpel, p=0.001). The number of cells present on group Bl was significantly higher than group Al (p=0.001). However at the 14th day there was no such difference (p=0,072). It is concluded that the small number of myofibroblasts at day eight after wounding with the CO_2 Lasermay be the reason why contraction on this wound is smaller than the one observed in conventional surgery.
机译：创伤性和手术性的伤口收缩都可能减少或限制组织的功能。肌成纤维细胞是涉及伤口收缩过程的细胞，在CO_2激光伤口上较小。这项研究的目的是定量和统计评估常规和CO_2激光伤口上肌成纤维细胞的存在。将32只动物（家兔）分为四组，并使用CO_2 Laser（A1和A2组）或常规手术刀（B1和B2组）进行手术。术后八天（组：A1和B1）和手术后第14天（组：A2和B2）处死动物。我们将标本常规加工成蜡，并用α-平滑肌肌动蛋白（aSMA）染色，并在光学显微镜（40X）下进行分析。选择每个载玻片伤口周围的两个标准区域，并使用校准的目镜和标线来计数存在的成肌纤维细胞的数量。第八天的肌成纤维细胞数量明显高于第14天。第八天两种技术的比较显示两组之间的显着差异（激光，p = 0.007和手术刀，p = 0.001）。 B1组中存在的细胞数量明显高于A1组（p = 0.001）。但是，在第14天没有这种差异（p = 0,072）。可以得出结论，在用CO_2激光创伤后的第八天，成肌纤维细胞数量很少，这可能是该伤口上的收缩小于常规手术中观察到的收缩的原因。

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