Development of a Flexible System for the Simultaneous Conversion of Biomass to Industrial Chemicals and the Production of Industrial Biocatalysts

机译：开发灵活的系统，用于将生物质同时转化为工业化学品和生产工业生物催化剂

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A flexible system was developed for the simultaneous conversion of biomass to industrial chemicals and the production of industrial biocatalysts. In particular, the expression of a bacterial enzyme, beta-glucuronidase (GUS), was investigated using a genetically modified starch-degrading Saccharomyces strain in suspension cultures in starch media. Different sources of starch, including corn and waste potato starch, were used for yeast biomass accumulation and GUS expression studies under controls of inducible and constitutive promoters. A thermostable bacterial cellulase (E1 beta-1,4-endoglucanase) gene of Acidothermus cellulolyticus was also cloned into an episomal plasmid expression vector and expressed in the starch-degrading Saccharomyces strain.

机译：开发了一种灵活的系统，用于将生物质同时转化为工业化学品和生产工业生物催化剂。特别地，使用转基因的降解淀粉的酵母菌菌株在淀粉培养基中的悬浮培养物中研究了细菌酶β-葡萄糖醛酸苷酶（GUS）的表达。在诱导型和组成型启动子的控制下，将淀粉的不同来源（包括玉米淀粉和马铃薯废淀粉）用于酵母生物量积累和GUS表达研究。也将解热嗜酸菌的热稳定细菌纤维素酶（E1 beta-1,4-endoglucananase）基因克隆到附加质粒表达载体中，并在降解淀粉的酵母菌株中表达。

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《Fourth Annual Green Chemistry and Engineering Conference Jun 27-29, 2000 Washington, D.C.》|2000年|p.145-161|共17页
会议地点 Washington DC(US);Washington DC(US)
作者
Johnway Gao; Brian S. Hooker; Rodney S. Skeen; Daniel B. Anderson;
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Environmental Technology Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, P.O. Box 999, MS K2-10, Richland, WA 99352;

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原文格式 PDF
正文语种 eng
中图分类环境科学基础理论;
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4. Development of a Flexible System for the Simultaneous Conversion of Biomass to Industrial Chemicals and the Production of Industrial Biocatalysts [C] . Johnway Gao, Brian S. Hooker, Rodney S. Skeen, International Workshop on Green Chemistry and Engineering . 2002

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5. Development of a system for characterizing biomass quality of lignocellulosic feedstocks for biochemical conversion. [D] . Murphy, Patrick Thomas. 2009

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7. Biomass Converting Enzymes as Industrial Biocatalysts for Fuels and Chemicals: Recent Developments [O] . Feng Xu, Matt D. Sweeney 2012

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Development of a Flexible System for the Simultaneous Conversion of Biomass to Industrial Chemicals and the Production of Industrial Biocatalysts

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