Arsenite Exposure Causes Both Hypomethylation and Hypermethylation in Human Cell Lines in Culture at Low Concentrations

机译：砷暴露导致低浓度培养的人细胞系中的低甲基化和高甲基化

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

We and others have hypothesized that a mechanism of arsenic carcinogenesis could involve alteration of DNA methylation since this process utilizes a methyltransferase and consumes S-adenosyl-methionine (SAM) as the methyl donor. We analyzed differentially methylated regions of genomic DNA from human lung A549 cells and three human kidney cell lines (UOK cell lines) after arsenite treatment using methylation-sensitive arbitrarily primed PCR (MS-APPCR). Relative to untreated controls, six DNA fragments identified by MS-APPCR were hypermethylated and two were hypo-methylated in DNA from these lung and kidney cell lines. Two of the DNA fragments contained repeat sequences of mammalian-apparent long terminal repeat retrotransposons (MaLRs), and five were identified to be promoter-like sequences. DNA methylation alterations were manifested at low nanomolar concentrations in some cell lines. The mRNA for DNA methyltransferase (MTase) increased in cells exposed to arsenite over a 2-week period. Our results show the occurrence of both hypermethylation and hypomethylation of DNA after exposure to arsenite. The results support the existence of a state of methylation imbalance (hypo- and hypermethylation) that could conceivably disrupt gene expression in arsenic exposed cells in vitro.

机译：我们和其他人假设砷致癌的机制可能涉及DNA甲基化的改变，因为该过程利用甲基转移酶并消耗S-腺苷甲硫氨酸（SAM）作为甲基供体。我们使用亚甲基化敏感的任意引物PCR（MS-APPCR）分析了亚砷酸盐处理后来自人肺A549细胞和三个人肾细胞系（UOK细胞系）的基因组DNA的差异甲基化区域。相对于未经处理的对照，通过MS-APPCR鉴定的六个DNA片段在这些肺和肾细胞系的DNA中被高甲基化，而两个则被低甲基化。其中两个DNA片段包含哺乳动物表观的长末端重复逆转录转座子（MaLRs）的重复序列，其中五个被鉴定为启动子样序列。在某些细胞系中，DNA甲基化改变表现在低纳摩尔浓度下。 DNA甲基转移酶（MTase）的mRNA在暴露于亚砷酸盐的细胞中持续2周的时间增加。我们的结果表明，暴露于亚砷酸盐后，DNA发生了甲基化过高和甲基化过低的现象。结果支持甲基化不平衡状态（低甲基化和高甲基化）的存在，该状态可能会破坏体外砷暴露细胞中的基因表达。

著录项

来源
《Fourth International Conference on Arsenic Exposure and Health Effects, 4th, Jun 18-22, 2000, San Diego, California》|2000年|p.243-254|共12页
会议地点 San Diego CA(US);San Diego CA(US)
作者
Cathy Zhong; Liangjun Wang; Marc J. Mass;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类 P6;
关键词
arsenic; DNA methylation; hypermethylation; hypomethylation;

机译：砷; DNA甲基化；甲基化低甲基化;

相似文献

外文文献
中文文献
专利

1. Both hypomethylation and hypermethylation of DNA associated with arsenite exposure in cultures of human cells identified by methylation-sensitive arbitrarily-primed PCR. [J] . Zhong CX, Mass MJ Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research . 2001,第3期

机译：通过对甲基化敏感的任意引物PCR鉴定的人类细胞培养物中与砷暴露相关的DNA的甲基化不足和甲基化过高。
2. Long-term low-dose exposure of human urothelial cells to sodium arsenite activates lipocalin-2 via promoter hypomethylation [J] . WangH.-H., WuM.-M., ChanM.W.Y., Archives of Toxicology . 2014,第8期

机译：人尿路上皮细胞长期低剂量暴露于亚砷酸钠可通过启动子低甲基化激活lipocalin-2
3. LINE-1 gene hypomethylation and p16 gene hypermethylation in HepG2 cells induced by low-dose and long-term triclosan exposure: The role of hydroxyl group [J] . Zeng Liudan, Ma Huimin, Pan Shangxia, Toxicology in vitro: an international journal published in association with BIBRA . 2016,第Null期

机译：低剂量和长期三氯生暴露引起的HepG2细胞LINE-1基因低甲基化和p16基因高甲基化：羟基的作用
4. Arsenite Exposure Causes Both Hypomethylation and Hypermethylation in Human Cell Lines in Culture at Low Concentrations [C] . L, Marc J. Mass, Liangjun Wang, International conference on arsenic exposure and health effects . 2001

机译：在低浓度下，砷酸盐暴露导致培养中的人细胞系中的低甲基化和高甲基化
5. Comparison of two human cell lines following exposure to low dose radiation and treatment with soybean miso and its active ingredient genistein. [D] . Sattler, Jennifer A. 2013

机译：两种人细胞系在低剂量辐射照射下以及大豆味soybean及其有效成分染料木黄酮处理后的比较。
6. Expression of hsp 27 hsp 60 hsc 70 and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite. [O] . Michael R Rossi, Seema Somji, Scott H Garrett, 2002

机译：热休克蛋白27热休克蛋白60热休克蛋白70和热休克蛋白70应激反应基因在暴露于致死和亚致死浓度的亚砷酸钠的培养的人尿道上皮细胞（UROtsa）中的表达。
7. Both hypomethylation and hypermethylation of DNA associated with arsenite exposure in cultures of human cells identified by methylation-sensitive arbitrarily-primed PCR [O] . C Zhong 2001

机译：与甲基化敏感的任意灌注PCR鉴定的人细胞培养物中的低甲基化和高甲基化的DNA含有与砷酸盐暴露相关的DNA
8. Near Infrared, High Energy, Ultrashort Pulse Laser-Light Exposure Genetically Induces p53, a Gene in the DNA Repair and Cell Suicide Pathways in Cultured Human Cells [R] . Obringer, J. W. , Phipps, S. , Johnson, M. D. 1999

机译：近红外，高能量，超短脉冲激光曝光基因诱导p53，DNa修复中的基因和培养的人类细胞中的细胞自杀途径

Arsenite Exposure Causes Both Hypomethylation and Hypermethylation in Human Cell Lines in Culture at Low Concentrations

摘要

著录项

相似文献

相关主题

期刊订阅