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首页> 外文会议>Frontiers in biological detection: from nanosensors to systems VI >Dual-mode lensless imaging device for digital enzyme linked immunosorbent assay
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Dual-mode lensless imaging device for digital enzyme linked immunosorbent assay

机译：用于数字酶联免疫吸附测定的双模式无透镜成像设备

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摘要

Digital enzyme linked immunosorbent assay (ELISA) is an ultra-sensitive technology for detecting biomarkers and viruses etc. As a conventional ELISA technique, a target molecule is bonded to an antibody with an enzyme by antigen-antibody reaction. In this technology, a femto-liter droplet chamber array is used as reaction chambers. Due to its small volume, the concentration of fluorescent product by single enzyme can be sufficient for detection by a fluorescent microscopy. In this work, we demonstrate a miniaturized lensless imaging device for digital ELISA by using a custom image sensor. The pixel array of the sensor is coated with a 20 μm-thick yellow filter to eliminate excitation light at 470 nm and covered by a fiber optic plate (FOP) to protect the sensor without resolution degradation. The droplet chamber array formed on a 50μm-thick glass plate is directly placed on the FOP. In the digital ELISA, microbeads coated with antibody are loaded into the droplet chamber array, and the ratio of the fluorescent to the non-fluorescent chambers with the microbeads are observed. In the fluorescence imaging, the spatial resolution is degraded by the spreading through the glass plate because the fluorescence is irradiated omnidirectionally. This degradation is compensated by image processing and the resolution of ～35 μm was achieved. In the bright field imaging, the projected images of the beads with collimated illumination are observed. By varying the incident angle and image composition, microbeads were successfully imaged.
机译：数字酶联免疫吸附测定法（ELISA）是一种用于检测生物标志物和病毒等的超灵敏技术。作为常规ELISA技术，靶分子通过抗原抗体反应通过酶与抗体结合。在该技术中，将飞升的液滴室阵列用作反应室。由于其体积小，单个酶产生的荧光产物的浓度足以用于荧光显微镜检测。在这项工作中，我们通过使用自定义图像传感器演示了用于数字ELISA的小型无透镜成像设备。传感器的像素阵列涂有厚度为20μm的黄色滤光片，以消除470 nm的激发光，并被光纤板（FOP）覆盖，以保护传感器而不会降低分辨率。在50μm厚的玻璃板上形成的液滴腔阵列直接放置在FOP上。在数字ELISA中，将涂有抗体的微珠装入微滴小室阵列，并观察带有微珠的荧光室与非荧光室的比例。在荧光成像中，由于荧光是全方位照射的，因此空间分辨率会因在玻璃板上的扩散而降低。这种退化可以通过图像处理得到补偿，分辨率达到〜35μm。在明场成像中，观察到具有准直照明的珠子的投影图像。通过改变入射角和图像组成，微珠被成功成像。

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  • 会议地点 San Francisco CA(US)
  • 作者

    Kiyotaka Sasagawa; Soo Hyeon Kim; Kazuya Miyazawa; Hironari Takehara; Toshihiko Noda; Takashi Tokuda; Ryota Iino; Hiroyuki Noji; Jun Ohta;

  • 作者单位

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 TakayamaIkoma Nara, JAPAN 630-0192,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

    The University of Tokyo, JAPAN,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 TakayamaIkoma Nara, JAPAN 630-0192;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 TakayamaIkoma Nara, JAPAN 630-0192;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 TakayamaIkoma Nara, JAPAN 630-0192,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 TakayamaIkoma Nara, JAPAN 630-0192,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

    The University of Tokyo, JAPAN,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

    The University of Tokyo, JAPAN,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

    Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 TakayamaIkoma Nara, JAPAN 630-0192,Core Research for EvolutionalScience and Technology (CREST), JST, JAPAN;

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  • 正文语种 eng
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  • 关键词

    digital enzyme-linked immunosorbent assay; CMOS image sensor; lensless imaging; fluorescent imaging; non-fluorescent imaging;

    机译：数字酶联免疫吸附试验CMOS图像传感器无透镜成像；荧光成像非荧光成像;

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