Single cell manipulation utilizing femtosecond laser-induced shock and stress waves

机译：利用飞秒激光诱发的冲击波和应力波进行单细胞操作

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When an intense femtosecond laser pulse is focused into a culture medium through an objective lens, an impulsive force is loaded on the cells with generations of the shock and stress waves at the laser focal point. The shock and stress waves were acted to single cells in the vicinity of the laser focal point as an impulsive force. We have applied the impulsive force to manipulate single cells. As the transient intensity of the impulsive force is over 1000 times stronger than the force due to optical tweezers, drastic single manipulation which is difficult by the optical tweezers can be realized. The generation process of the impulsive force and behavior of animal cell after loading the impulsive force were reviewed, and then our original quantification method of the impulsive force utilizing atomic force microscope (AFM) was introduced with its applications for evaluating adhesions between animal cells and between sub-organelles in plant cell.

机译：当飞秒的强激光脉冲通过物镜聚焦到培养基中时，脉冲力会在激光焦点处产生冲击波和应力波，从而施加到细胞上。冲击波和应力波作为脉冲力作用于激光焦点附近的单个单元。我们已经施加了脉冲力来操纵单个细胞。由于脉冲力的瞬态强度比由光镊引起的力强1000倍以上，因此可以实现光镊难以进行的剧烈的单次操作。回顾了脉冲力加载后动物细胞脉冲力的产生过程和行为，然后介绍了我们最初利用原子力显微镜（AFM）对脉冲力进行量化的方法，并将其用于评估动物细胞之间和动物之间的粘附力。植物细胞中的亚细胞器。

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《Frontiers in ultrafast optics: biomedical, scientific, and industrial applications XVII》|2017年|100940A.1-100940A.6|共6页
会议地点 San Francisco(US)
作者
Yoichiroh Hosokawa;
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作者单位

Graduate School of Materials Science, Nara Institute of Science and Technology 8916-5 Takayama, Ikoma, Nara, JAPAN 630-0192;

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会议组织
原文格式 PDF
正文语种 eng
中图分类
关键词
laser and microscope; biotechnology; optical tweezers; atomic force microscope; optical breakdown; Shockwave; cavitation bubble; animal cell; plant cell;

机译：激光和显微镜生物技术光学镊子；原子力显微镜光学击穿冲击波空化气泡动物细胞植物细胞;

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2. Contribution of stress wave and cavitation bubble in evaluation of cell-cell adhesion by femtosecond laser-induced impulse [J] . Takanori Iino, Po-Lin Li, Wen-Zhe Wang, Applied Physics . 2014,第1期

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3. Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave [J] . Yung-En Kuo, Cheng-Chi Wu, Yoichiroh Hosokawa, Applied Physics . 2010,第4期

机译：飞秒激光诱导的应力波对培养的心肌细胞的局部刺激
4. Single cell manipulation utilizing femtosecond laser-induced shock and stress waves [C] . Yoichiroh Hosokawa Conference on frontiers in ultrafast optics: biomedical, scientific, and industrial applications XVII . 2017

机译：利用飞秒激光诱导的冲击和应力波的单细胞操纵
5. Applications of capillary electrophoresis and laser-induced fluorescence detection to the analysis of trace species: From single cells to single molecules. [D] . Xue, Qifeng. 1995

机译：毛细管电泳和激光诱导荧光检测在痕量物质分析中的应用：从单个细胞到单个分子。
6. The influence of laser-induced nanosecond rise-time stress waves on the microstructure and surface chemical activity of single crystal Cu nanopillars [O] . G. Youssef, R. Crum, S. V. Prikhodko, -1

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7. Single living cell processing in water medium using focused femtosecond laser-induced shockwave and cavitation bubble [O] . Hosokawa Y, Yasukuni R, Spitz Jean-Alexis, 2007

机译：利用聚焦飞秒激光诱导的冲击波和空化气泡处理水介质中的单个活细胞

Single cell manipulation utilizing femtosecond laser-induced shock and stress waves

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