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Biological Clogging in Compacted Mixtures of Ottawa Sand and Kaolinite

机译:渥太华砂和高岭石密实混合物的生物阻塞

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摘要

Biological clogging of porous media by metabolically active bacteria is an important issue in Geoenvironmental engineering. In this paper, the role of two important factors influencing biological clogging is evaluated. Results are presented from two different series of percolating experiments: 1) experiments conducted with sterile and unsterile soil, 2) experiments conducted with soils subjected to low and high hydraulic head. A mixture of Ottawa sand and Kaolinite was used as the porous medium and Pseudomonas aeruginosa as the bacterial culture. Results indicated that filtering as well as clogging by bacteria occurred in soil samples, as reflected by the reduction in the CFUs (Colony Forming Units) of the effluent compared to the influent CFUs. Comparisons of pore size distributions (PSD) of the soil before and after clogging revealed that both biomass growth and biogas generation were responsible for the permeability reduction. Experiments conducted with sterile and unsterile soil revealed that higher reduction in permeability could be achieved in unsterile soil because of the presence of indigenous soil microbes. Experiments conducted with sample subjected to high hydraulic head revealed that high heads would result in biofilm rupture formed on the surface, which would result in subsequent increase in permeability.
机译:代谢活性细菌对多孔介质的生物堵塞是地球环境工程中的一个重要问题。本文评估了影响生物堵塞的两个重要因素的作用。结果来自两个不同系列的渗滤实验:1)用无菌和非无菌土壤进行的实验,2)用承受低和高水头的土壤进行的实验。渥太华砂和高岭石的混合物用作多孔介质,铜绿假单胞菌用作细菌培养物。结果表明,土壤样品中发生了细菌的过滤和堵塞,这反映在废水中CFU(菌落形成单位)与进水CFU相比有所减少。比较堵塞前后土壤的孔径分布(PSD),发现生物量的增长和沼气的产生均与渗透率降低有关。在无菌和非无菌土壤上进行的实验表明,由于存在土著土壤微生物,因此在无菌土壤中可以实现更高的渗透率降低。对承受高水头的样品进行的实验表明,高水头会导致表面上形成生物膜破裂,从而导致渗透率随后增加。

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