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Picosecond 3D fluorescence and absorption spectroscopy of a manipulated microparticle

机译：皮秒的3D荧光和吸收光谱

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Abstract: Dynamic microspectroscopy systems have been developed for elucidating photophysical and photochemical processes occurring in small volumes. The first system concerns fluorescence spectroscopic measurements based on a time-correlated single photon counting and a confocal fluorescence microscope, which makes it possible to observe the fluorescence dynamics with both picosecond time-resolution and micrometer three-dimensional space-resolution. The second system is for absorption spectroscopic measurements. Picosecond pump and probe beams are coaxially and confocally introduced into a microscope, giving the depth profile in addition to lateral information. These systems are further combined with a trapping laser which optically tweezers a single microparticle against its Brownian motion. Laser trapping thus introduces the precise time- and space-resolved measurement of particles. To analyze rise and decay curves as a function of position and wavelength, a new fast and accurate analyzing method using a convolved autoregressive model has been proposed. The computation time of this method is much shorter than that of the conventional methods. The present systems have been applied to various kinds of microparticles such as microcapsules, polymer latexes, and oil droplets.!22
机译：摘要：已经开发出动态显微光谱系统，以阐明小体积发生的光物理和光化学过程。第一个系统涉及基于时间相关的单光子计数和共聚焦荧光显微镜的荧光光谱测量，这使得可以用皮秒时间分辨率和千分尺三维空间分辨率观察荧光动力学。第二个系统用于吸收光谱测量。皮秒泵浦光束和探棒光束同轴共凹地引入显微镜，除提供横向信息外，还提供深度轮廓。这些系统还与捕集激光器结合，该捕集激光器对单个微粒的布朗运动进行光学钳制。因此，激光俘获引入了精确的时间和空间分辨的颗粒测量。为了分析作为位置和波长的函数的上升和下降曲线，提出了一种新的使用卷积自回归模型的快速准确的分析方法。该方法的计算时间比常规方法的计算时间短得多。本系统已经应用于各种微粒，例如微胶囊，聚合物胶乳和油滴。22

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