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首页> 外文会议>ICO20: Optical Devices and Instruments >Construction of a Fourier-Transform Phase-Modulation Fluorometer
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Construction of a Fourier-Transform Phase-Modulation Fluorometer

机译：傅里叶变换调相荧光计的构建

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We have constructed a Fourier-transform phase-modulation fluorometer (FT-PMF) by which a fluorescence decay waveform can be obtained. In the FT-PMF, the modulation frequency of the excitation light source is swept continuously from a direct current (dc) to a high frequency f_(max)with a time duration T. The resultant fluorescence signal waveform is Fourier-transformed to obtain its amplitude and phase spectra. The ratio of the amplitude spectrum and the difference of the phase spectrum over those of the reference spectra that are obtained from a non-fluorescent material are calculated, respectively, and the pair of both spectral data is inverse-Fourier-transformed again to obtain the fluorescence decay waveform. The light source used was an ultraviolet light emitting-diode (UV LED) whose typical operating condition was f_(max) = 100 MHz and T = 10 μs. To demonstrate the performance of the FT-PMF, we carried out (1) measurement of a fluorescent decay waveform of YAG materials packed in a white LED, and (2) determination of fluorescence lifetime of 10 ppm quinine sulfate in 0.1N H_2SO_4.
机译：我们构建了傅立叶变换相位调制荧光计（FT-PMF），通过它可以获得荧光衰减波形。在FT-PMF中，激发光源的调制频率从直流电（dc）连续扫描到持续时间为T的高频f_（max）。所得荧光信号波形经过傅立叶变换以获得其荧光信号波形。振幅和相位频谱。分别计算从非荧光材料获得的振幅光谱和相光谱与参考光谱的比率之差，并再次对这两个光谱数据进行傅立叶逆变换，以获得荧光衰减波形。使用的光源是紫外线发光二极管（UV LED），其典型工作条件为f_（max）= 100 MHz和T = 10μs。为了证明FT-PMF的性能，我们进行了（1）测量装在白色LED中的YAG材料的荧光衰减波形，以及（2）确定在0.1N H_2SO_4中10 ppm硫酸奎宁的荧光寿命。

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